金针菇固体液化菌种研究

Study on the solid liquefaction spawn of Flammulina velutipes

[目的]为探究新型菌种,推进食用菌工厂化生产的进程。[方法]首先对金针菇固体液化菌种的最佳培养基进行了筛选,之后,对金针菇固体液化菌种和常规液体菌种的菌丝形态、菌丝球浓度、还原糖含量、基质降解酶活力等指标的变化情况进行了比较与分析。[结果]金针菇固体液化菌种最佳原料为木屑粉。金针菇固体液化菌种菌球浓度在第6天达到最大值68个·mL^(-1),且菌球浓度远高于常规液体菌种。金针菇固体液化菌种还原糖含量在第4天达到最大值18.626g·L^(-1),比常规液体菌种提前1天;蛋白酶、纤维素酶和漆酶的活力均在第5天达到最大值后趋于平衡,且培养期间均高于常规液体菌种。[结论]固体液化菌种在菌球数量、基质降解活力和稳定性等方面明显优于对照常规液体菌种。

[Objective]The aim of this study was to develop new species,and to promote edible fungus factory production process[Methods]The best solidmaterials of liquefaction spawn of Flammulina velutipes were investigated.The changes of mycelial morphology,the concentrationcontent of hypha ball,concents of reducing sugar and substrate degradation enzyme activities of solid liquefaction and conventio nal liquid spawns of F.velutipes were compared and analyzed.[Result]The results showed that the best solidmaterials of solid liquefaction spawn of F.velutipes was wood flour.The concentration of hypha balls of liquefaction spawns of F.velutipes reached a maximum of 68ball/mL on the sixth day,and were still far above than the conventional liquid spawn.Contents of reducing sugars of solid liquefaction spawn of F.velutipesinc reased the maximum of 18.62mg/ml on the fourth day about one days earlier than the conventional liquid spawn.The activities of protease,cellulase and laccase of solid liquefaction spawn of F.velutipes were higher than the conventional liquid spawn before reaching the maximums and then droping down on the fifth day.[Conclusion]The concentrations of hypha ball,substrate degradation activities and stabilities of solid liquefaction spawns of F.velutipeswere were better than the control conventional liquid spawns.