^(60)Co-γ辐射石斛兰组培苗的SRAP分析

SRAP Analysis of Dendrobium Tissue Culture Seedlings Based on ^(60)Co-γ-ray Radiation

为研究石斛兰辐射诱变后的植株性状遗传变异程度,本研究利用SRAP分子标记对不同剂量^(60)Co-γ射线处理后的石斛兰D.soni‘a166’品种组培苗进行分析,探讨其辐射诱变效应,确定最佳的辐射剂量。结果表明:利用从100对引物中筛选出的12对引物对不同剂量处理的石斛兰基因组DNA进行扩增,共扩增出了216条带,其中多态性带41条,多态率为19.0%。对不同处理及对照扩增的谱带进行聚类分析发现,各个剂量的辐照处理群体都发生了不同程度的变异,20 Gy处理整体变异程度最大,5 Gy变异最小。因此,认为石斛兰D.soni‘a166’品种组培苗的最佳辐射诱变剂量为20 Gy。

To study the genetic variation degree of plant characters of Dendrobium tissue culture seedlingstreated by radiation mutation, in this study, SRAP molecular markers technique was used to analyze Dendrobium sonia‘166＇variety tissue culture seedlings treated by different doses of ^60Coγ ray. The radiationmutagenesis effect was discussed, and the optimal irradiation dosage was determined. The results showed that12 primers screened out from 100 pairs primers were used to amplify the genomic DNA of Dendrobium treatedwith different doses of ^60Coγ ray. The 216 bands were amplified, among them 41 bands were polymorphic withthe ratio of 19.0%. The cluster analysis of the bands amplified in treated and controlled plants showed that D.sonia‘166＇varieties tissue culture seedlings treated by different doses of ^60Coγ ray mutated in different extent.The variation degree was maximum in 20 Gy treated plants, and was minimum in 5 Gy treated plants. Therefore 20 Gy was the best radiation dose of the ^60Coγ-ray for variation induction.