短肽型肠内营养制剂对肠黏膜炎大鼠营养状况的影响

Effect of peptide-based enteral nutrition on nutritional state in intestinal mucositis rat

目的:探讨短肽型肠内营养制剂(PBEN)对肠黏膜炎大鼠营养状况的影响。方法:利用甲氨喋呤(MTX)建立大鼠肠黏膜炎模型。60只大鼠随机分为6组,即基础饲料(BF)组,短肽型肠内营养制剂(PBEN)组,整蛋白型肠内营养制剂(IPEN)组,MTX+BF组,MTX+PBEN组和MTX+IPEN组。于第0天和第6天,给予MTX+BF组、MTX+PBEN组和MTX+IPEN组腹腔注射10 mg/kg MTX造成大鼠肠道持续损伤。从第1天开始,BF组和MTX+BF组饲喂BF;PBEN组和MTX+PBEN组饲喂PBEN;IPEN组和MTX+IPEN组饲喂IPEN。每组大鼠每天按159.0 k J(38 kcal)/100 g给予膳食。每天记录各组大鼠的体重和进食量。于第11天处死大鼠,检测血浆中D-乳酸、二胺氧化酶(DAO)和血清总蛋白(TP)、清蛋白(ALB)、前清蛋白(PA)、转铁蛋白(TF)和视黄醇结合蛋白(RBP)水平。结果:大鼠进食BF或PBEN、IPEN后各指标间无显著性差异(P>0.05)。MTX造模导致大鼠肠黏膜严重损伤,MTX+BF组各项指标与BF组比有显著性差异(P<0.05)。MTX+PBEN组大鼠体重下降程度低于MTX+BF组和MTX+IPEN组(P<0.05)。第11天时,MTX+PBEN组大鼠血浆D-乳酸和DAO水平均低于MTX+BF组和MTX+IPEN组,且血清TP、ALB、PA、TF和RBP水平均高于MTX+BF组和MTX+IPEN组(P<0.05)。结论:PBEN可有效地改善肠黏膜炎大鼠的营养状况。

Objective： To evaluate the effects of peptide-based enteral nutrition （PBEN） on nutritional state in intestinal mucositis rats. Methods： The model of continuous intestinal mucosal injury was induced by intraperitoneal injection of methotrexate. 60 Sprague Dawley male rats were divided into six groups randomly ： basal feed （BF） group, peptide-based enteral nutrition （ PBEN ） group, intacted protein enteral nutrition（IPEN） group, MTX ＋ BF group, MTX ＋ PBEN group and MTX ＋ IPEN group. SD rats of MTX ＋ BF group, MTX ＋ PBEN group and MTX ＋ IPEN group were intraperitoneal injected with methotrexate 10 mg/kg respectively on day 0 and day 6. From day 1, BF group and MTX ＋ BF group were fed with basic food, PBEN group and MTX ＋ PBEN group were fed with PBEN, IPEN group and MTX ＋ IPEN group were fed with IPEN. The daily energy of each rat was given as 38 kcal/100 g per day. All animals were sacrificed on day 11 to observe the change of physiological and biochemical indexes, include the content of D-lactate and the activity of Diamine oxidase （DAO） in plasma, and the content of Total protein （TP）, Albumin （ALB）, prealbumin （PA）,Transferrin （RF）, Retinol binding protein （RBP） in serum. Results： There were no difference among BF group, PBEN group and IPEN group （P 〉 0.05 ）. MTX caused serious injury of intestinal tissue, and there were significant difference amongBF group and MTX ＋ BF group of each index （ P 〈 0.05 ）. The extent of reduction degree of in body weight of MTX ＋ PBEN group were significantly lower than MTX ＋ BF group and MTX ＋ IPEN group （ P 〈 0.05 ）. The level of D-lactate, DAO in plasma of MTX ＋ PBEN group were significantly lower than MTX ＋ BF group and MTX ＋ IPEN group （ P 〈 0.05 ） on day 11, the levels of TP, ALB, PA, TRF, RBP in serum were significantly higher than MTX ＋ BF group and MTX ＋ IPEN group （ P 〈 0.05 ）. Conclusion： PBEN can improve the nutritional state in intestinal mucositis rat.