应用cDNA-SRAP标记比较苹果梨与延光梨果皮性状的表达差异

Analysis of gene expression difference of peel characters between Pingguoli and Yanguangli by cDNA-SRAP markerss

以苹果梨和延光梨果皮为试材,对盛花后7周的苹果梨和延光梨果皮cDNA进行SRAP-PCR检测,找出2个梨品种(系)在转录水平的差异,共扩增出444条可被应用的条带。2个品种间的相似性极高,仅检测到2个差异片段,多态性为0.45%。其中,ME7-EM5引物组合扩增出的差异条带是因为核苷酸序列包含内含子序列,为模板中存在的少量DNA扩增;ME4-EM3引物组合扩增出的差异条带经回收测序,于Blast上比对得知其为植物凝集素基因,经半定量RT-PCR验证多态性消失,判断差异条带可能是由于苹果梨与延光梨引物结合位点存在差异而致。

Make the peel of Pingguoli and Yanguangli at 7 weeks after full bloom as materials, the difference between the two pear cultivar＇s （lines） was determined by using cDNA-SRAP technology at the level of transcription. As the result, 444 bands were amplified from cDNAs of the peels of Pingguoli and Yanguangli with only 2 different bands. The two cultivars were highly related with low polymorphism of 0. 45%. The 2 polymorphic bands were amplified with primer combinations of MET-EM5 and ME4-EM3. The polymorphic bands amplified with MET-EM5 primer combination resulted from the introns involved, whereas the polymorphic band amplified with ME4-EM3 primer combination was a partial fragment of Lectin gene revealed by blast.