人类免疫缺陷病毒/获得性免疫缺陷综合征肺脾气虚证的病理机制研究

Pathological Mechanism of Lung Spleen Qi Deficiency Syndrome in HIV/AIDS

目的探讨人类免疫缺陷病毒(HIV)/获得性免疫缺陷综合征(AIDS)肺脾气虚证患者载脂蛋白(Apo)AⅠ、Apo AⅡ、Apo B、锌α2-糖蛋白(AZGP1)表达水平的差异,为研究HIV/AIDS中医证候提供依据。方法2013年12月—2014年6月选取河南省某地区的HIV/AIDS肺脾气虚证患者28例为HIV/AIDS肺脾气虚证组(FP组)。同期选取同地区健康人16例为对照组(JK组)。采集研究对象血清,检测CD_4、CD_8水平及CD_4/CD_8,采用i TRAQ-MS技术鉴定Apo AⅠ、Apo AⅡ、Apo B、AZGP1水平,酶联免疫吸附试验(ELISA)法检测Apo AⅠ、Apo AⅡ、Apo B、AZGP1水平。结果 FP组CD_4水平低于JK组,CD_4/CD_8小于JK组(P<0.05);两组CD_8水平比较,差异无统计学意义(P>0.05)。两组i TRAQ-MS技术鉴定Apo AⅠ水平比较,差异无统计学意义(P>0.05);FP组i TRAQ-MS技术鉴定Apo AⅡ水平高于JK组,i TRAQ-MS技术鉴定Apo B、AZGP1水平低于JK组(P<0.05)。两组ELISA法检测Apo AⅠ水平比较,差异无统计学意义(P>0.05);FP组ELISA法检测Apo AⅡ水平高于JK组,ELISA法检测Apo B、AZGP1水平低于JK组(P<0.05)。结论 HIV/AIDS肺脾气虚证患者血清中Apo AⅡ水平升高,Apo B、AZGP1水平降低,与脂质代谢有关。

Objective To explore the difference of expression levels of ApoA I , ApoA 11 , ApoB and AZGP1 among patients with lung spleen qi deficiency syndrome and HIV/AIDS, and provide the basis for research of TCM syndrome features of HIV/AIDS. Methods From December 2013 to June 2014, 28 HIV/AIDS patients with lung spleen qi deficiency syndrome from a certain area of He＇nan province were selected as HIV/AIDS with lung spleen qi deficiency syndrome group （ FP group） . In the same period, 16 healthy people from the same region were selected as control group （JK group） . Serum of objects was collected, and the levels of CD4 , CDs and CDJCDs were detected. The levels of ApoA I , ApoA II , ApoB, AZGP1 were identified by iTRAQ - MS technology, and detected by ELISA method. Results The level of CD4 and CD4/CDs in FP group were significantly lower than those in JK group （ P 〈 O. 05 ）. There was no significant difference in CDs level between the two groups （ P 〉 0. 05 ）. There was no significant difference in ApoA I level identified by iTRAQ - MS technology between the two groups （ P 〉 0. 05 ）. The ApoA I1 level identified by iTRAQ - MS technology in FP group was higher than that in JK group （ P 〈 0. 05 ） . Levels of ApoB and AZGP1 identified by iTRAQ - MS technology in FP group were lower than those in JK group （ P 〈 0. 05 ） . There was no significant difference in ApoA I level detected by ELISA method between the two groups （ P 〉 0. 05 ） . The ApoA 11 level detected by ELISA method in FP group was higher than that in JK group （ P 〈 0. 05 ） . Levels of ApoB and AZGP1 detected by ELISA method in FP group were lower than those in JK group （ P 〈 O. 05 ） . Conclusion ApoA I1 level increased, levels of ApoB and AZGP1 decreased in serum of HIV/AIDS patients with lung spleen qi deficiency syndrome, and this is related to lipid metabolism.