人子宫颈癌基因在子宫颈上皮内瘤变和子宫颈鳞癌中的表达及其与HPV16整合状态的关系

Expression of HCCR in cervical intraepithelial neoplasia and cervical carcinoma and its relationship with HPV16 infection

目的探讨人宫颈癌基因(human cervical cancer oncogene,HCCR)在宫颈上皮内瘤变(CIN)和宫颈鳞癌(SCC)中的表达及其与HPV16整合状态的关系。方法选取2013年1月-2015年3月收治的HPV16阳性宫颈炎患者20例、CIN患者44例和SCC患者20例。采用多重实时PCR技术检测宫颈脱落细胞中的E2和E6基因,通过E2/E6比值,评估HPVl6型DNA的体内整合状态。采用免疫组化SP法测定各组患者宫颈脱落细胞HCCR表达情况。结果宫颈炎、CIN和SCC组HPV16 DNA游离型分别是95.00%(19/20)、43.18%(19/44)和15.00%(3/20),HPV16 DNA混合型分别是5.00%(1/20)、50.00%(22/44)和55.00%(11/20),HPV16 DNA整合型分别是0、6.82%(3/44)和30.00%(6/20),各组间比较,差异有统计学意义(P<0.05);在HPV16 DNA游离型及混合型组患者中,宫颈炎、CIN和SCC组HCCR表达逐渐增加,差异有统计学意义(P<0.05)。SCC组HPVl6DNA整合状态与年龄、临床期别、分化程度及淋巴结转移无关。结论 HPV感染可能与HCCR及HPV16整合状态有关,HCCR及HPV16存在状态检测可能作为细胞学筛查的一种补充手段,以期早发现CIN及SCC的高危患者。

Objective To investigate the expression of human cervical cancer oncogene（HCCR） in cervical intraepithelial neoplasia （CIN） and cervical carcinoma and its relationship with HPV16 infection. Methods 84 cases including 44 cases with CIN 20 cases with cervical cancer and 20 cases with cervicitis who were treated in our department from January 20 13 to march 2015,reeruited in this study.Cervical liquid-based cytological samples were collected to detect E2 and E6 genes of HPV type 16 by using multiple real PCR time polymerase chain reaction（PCR）. E2/E6 ratio was used to evaluate the physical status of HPV16 DNA in host cell genome. The SP immunohistochemical method was used to detect the expression of HCCR in cervical lesions. Results The expression of free type of HPV16 DNA in cervicitis,CIN and SCC was 95%,43.18% and 15%,the expression of hybrid type of HPV16 DNA in cervicitis,CTN and SCC was 5%,50% and 55%, the expression of integrated type of HPV16 DNA in cervicitis,CIN and SCC was 0, 6.82% and 30%.The difference was statistically significantJn the group of free and hybrid type of HPV16 DNA, the expression of HCCR grew with the disease progression. In SCC, HPV16 DNA exists status had nothing to with age, clinical stage, differentiation, lymph node metastasis. Conclusion The infection of HPV is related to the status of HCCR and HPV16, which may be a additional method for the screening of cervical cancer.