高效液相色谱法分析武夷岩茶中的生物碱含量

The analysis of alkaloid content in Wuyi Rock Tea with high-performance liquid chromatography

选取10种典型武夷岩茶(5种肉桂和5种水仙)作为研究对象,以绿茶、铁观音和红茶为对照,通过高效液相色谱法检测样品叶片及冲泡茶汤中的可可碱、茶碱和咖啡碱含量,并依据各样品的生物碱含量进行主成分分析。试验结果发现,武夷岩茶叶片中的咖啡碱、可可碱和茶碱含量分别约占茶叶干重的2.341%,0.065%,0.011%,低于绿茶、铁观音和红茶;茶叶经过60s沸水冲泡后,约46.56%,46.36%,50.15%的咖啡碱、可可碱和茶碱溶于茶汤中。此外,通过主成分分析发现,武夷岩茶的3种生物碱含量与绿茶、铁观音和红茶的生物碱含量有显著差异。

Wuyi Rock Tea is a kind of semi-fermented oolong tea, with unique flavor. The alkaloid content in the tea is associated with its flavor quality. In this study, 10 typical Wuyi Rock Tea, including 5 Rougui and 5 Shuixian, were used to quantitative analyze the theo- bromine, theophylline and caffeine content in the tea leaves and tea infusions. Moverover, the alkaloid content in green tea, Tieguanyin Tea and black tea sample were also measured. Then, Principal Com- ponent Analysis （PCA） was utilized to further investigate the differ- ence of the alkaloid content in all these tea samples. The results indi- cated that, the caffeine, theobromine and theophylline content in the Wuyi Rock Tea leaves were 2.341%, 0.065% and 0.011%, respec- tively, which were lower than those ingreen tea, Tieguanyin Tea and black tea. After immersed in boiling water for 60 s, approximate 46.56%, 46.36% and 50.15%of caffeine, theobromine, and theo- phylline were dissolved into the infusions. PCA analysis shown thatWuyi Rock Tea samples had significantly different alkaloid profile with green tea, Tieguanyin Tea and black tea.