运动和罗格列酮降低ADMA的协同作用及其机制研究

Synergistic Effect and Mechanism of Exercise and Rosiglitazone on Reducing ADMA

目的:研究运动与罗格列酮联合对T2DM大鼠ADMA水平的影响,观察其是否存在协同效应。方法:采用高质饮食结合腹腔注射低剂量STZ的方法建立T2DM大鼠模型,将实验动物分为正常对照组、T2DM组、T2DM+运动组、T2DM+罗格列酮组和T2DM+运动+罗格列酮组,每组给予相应的干预10周。结果:罗格列酮可以降低T2DM大鼠CML(P<0.01)和ADMA(P<0.01),同时上调肝脏PPAR-γ(P<0.01)和DDAH-1(P<0.01)mRNA表达,而运动可以降低T2DM大鼠CML(P<0.01)和ADMA(P<0.05),同时上调肝脏PPAR-γ(P<0.05)和DDAH-1(P<0.01)mRNA表达;运动和罗格列酮联合干预组CML明显低于罗格列酮单独干预(P<0.01),ADMA明显低于单独运动干预(P<0.05),肝脏PPAR-γ和DDAH-1mRNA表达分别显著高于单独运动((P<0.01)和单独罗格列酮(P<0.01)干预。结论:运动和罗格列酮在降低ADMA保护血管内皮方面可能存在协同效应,其机制可能是基于AGEs/PPAR-γ/DDAH/ADMA途径。

To explore effect of exercise and Rosiglitazone on reducing ADMA,is there the presence of synergistic effect？STZ induced T2 DM rats model were divided into control group,T2 DM group,T2DM＋Rosiglitazone group,T2DM＋Exercise group,and T2DM＋Rosiglitazone＋ Exercise group,for 10 weeks intervention.The results show that Rosiglitazone can reduce the level of CML（P〈0.01）and ADMA（P〈0.01）,and increase the liver ppar-gamma（P〈0.01）and DDAH-1（P〈0.01）mRNA expression compared with T2 DM group.Exercise can reduce the level of CML（P〈0.01）and ADMA（P〈0.05）,and increase the liver ppar-gamma（P〈0.05）and DDAH-1（P〈0.01）mRNA expression compared with T2 DM group.Exercise and Rosiglitazone combined together reduce the level of CML significantly lower than single Rosiglitazone group（P〈0.01）,ADMA significantly lower than the single exercise group（P〈0.05）,liver ppar-gamma and DDAH-1 mRNA expression were significantly higher than exercise group（P〈0.01）and Rosiglitazone group（P〈0.01）separately.In conclusion,there is possible synergistic effect of exercise and Rosiglitazone on reducing ADMA,its mechanism based on AGEs/PPAR-gamma/DDAH/ADMA pathway.