小细胞肺癌组织中LncRNA AK09398的表达及与预后的关系研究

Expression of LncRNA AK09398 and Relationship with Prognosis in Patients with Small Cell Lung Cancer

背景与目的小细胞肺癌(small cell lung cancer,SCLC)约占肺癌的15%,由于较早发生血行及淋巴转移,SCLC患者的预后差,其具体机制尚不明确。研究发现Lnc RNA(noncoding RNA,nc RNA)在肿瘤的发生、发展、转移、凋亡中起着重要的作用。本研究旨在探讨Lnc RNA AK09398在SCLC组织标本中的表达及临床意义。方法通过q RT-PCR法检测Lnc RNA AK09398在118例临床资料完整的SCLC肺癌组织及正常肺组织标本中的表达,分析其表达与患者临床病理特征及预后的关系。结果 Lnc RNA AK09398在肺癌癌组织中的平均表达水平为(7.813±0.373),与癌旁组织(1.782±0.116)及正常肺组织(1.209±0.200)比较,差异有统计学意义(F=58.41,P<0.001)。Lnc RNA AK09398在SCLC组织标本中的表达较癌旁组织明显增高。Lnc RNA AK09398的表达与患者的年龄、性别无关,差异无统计学意义(P均>0.05);与疾病分期、淋巴结转移及远处转移、化疗敏感性及生存状态明显相关,差异有统计学意义(P均<0.05)。高表达Lnc RNA AK09398患者的总生存时间及无进展生存时间均较低表达者明显缩短;Cox多因素回归模型分析提示,Lnc RNA AK09398的表达、远处转移及临床分期是SCLC独立的预后因素(P<0.05)。结论 Lnc RNA AK09398参与调节SCLC的发生发展,可能作为潜在的SCLC预后评估的分子标志物。

Background and objective Small cell lung cancer（SCLC） accounts for approximately 15% of lung cancer. The poor prognosis of SCLC patients is attributed to the early blood and lymph node metastasis. However, the mechanism of SCLC is still unclear. Recent studies show that Lnc RNA plays an important role in the development, metastasis, and apoptosis of tumor. Thus, the present study aimed to investigate the expression and clinical significance of Lnc RNA AK09398 in SCLC. Methods Quantitative reverse transcription polymerase chain reaction（q RT-PCR） was used to detect the expression of Lnc RNA AK09398 in 118 cases of SCLC and non-cancerous tissues. q RT-PCR was also utilized to analyze the relationship between the expression and clinical pathological characteristics and prognosis of SCLC. Results The expression of Lnc RNA AK09398 in lung cancer tissues was（7.813±0.373）. This value was significantly increased in paracarcinoma tissues（1.782±0.116） and normal lung tissues（1.209±0.200）. The difference was statistically significant（F=58.41, P〈0.001）. AK09398 expression was positively correlated with disease stage, lymph node and distant metastasis, chemosensitivity, and survival status（P〈0.05）. However, AK09398 expression was not associated with gender and age（P〈0.05）. Kaplan-Meier analysis demonstrated that high expression level of AK09398 contributed to poor overall survival（P〈0.001） and progression-free survival（P〈0.001）. The multivariate survival analysis also indicated that AK09398 expression, disease stage, and distant metastasis could be independent prognostic markers. Conclusion Lnc RNA AK09398 is involved in the development of SCLC and can be used as a molecular marker to evaluate the prognosis of SCLC.