黄芩苷对过氧化氢引起新生大鼠原代海马神经细胞氧化损伤的保护作用

Protective effects of Baicalin on the H_2O_2 injured hippocampal nerve cell in neonatal rats

目的研究黄芩苷(BCL)对过氧化氢(H_2O_2)引起新生大鼠原代海马神经细胞氧化损伤的保护作用。方法分离新生大鼠海马神经细胞并进行原代培养,然后将200μmol·L^(-1)H_2O_2加入到3个质量浓度(25,50,100 mg·m L^(-1)BCL)预处理2 h的细胞培养液中,培养24 h后,以四甲基偶氮唑盐(MTT)法检测细胞存活率;用试剂盒测定培养液内超氧化物歧化酶(SOD)的活力、丙二醛(MDA)的含量以及乳酸脱氢酶(LDH)的释放量。结果模型组的细胞存活率(77.01%)与空白组的细胞存活率(100%)比较,差异有统计学意义(P<0.05);低中高3个量质量浓度实验组共同孵育24 h后的细胞存活率与模型组相比均有所增加,中、高2个质量浓度实验组的BCL的细胞存活率分别为87.00%和94.12%,组间比较差异有统计学意义(P<0.05)。高质量浓度实验组的SOD活力为(32.98±6.13)U·m L^(-1),较模型组的(23.56±4.19)U·m L^(-1)明显升高,差异有统计学意义(P<0.05)。中高2个质量浓度实验组的MDA含量分别为(2.98±0.59),(2.81±0.63)nmol·m L^(-1),较模型组的MDA含量(3.96±0.79)nmol·m L^(-1)均显著降低,差异有统计学意义(P<0.05)。中高2个质量浓度实验组的LDH释放量分别为(334.58±101.41),(327.83±92.66)U·L^(-1),较模型组的LDH释放量(519.67±103.78)U·L^(-1)显著降低,差异有统计学意义(P<0.01)。结论 BCL对H_2O_2引起的新生大鼠原代海马神经细胞的氧化应激损伤具有一定的保护作用。

Objective To investigate the protective effects of Baicalin （BCL）on the 2O2 -injured hippocampal nerve cell in neonatal rats. Methods Different concentration （25, 50, 100 mg . mL-1） of BCl were joined in hippocampal nerve cell in neonatal rats within cultures for 2 h, then 200 μmol . L-12O2 was joined. Then 24 h later, the survival rate of cells was detected by MTI＇. The activity of SOD, the content of MDA and the leaking of LDH in the cultures were examined by related kits. Results The survival rate of ceils （77.01%） in model group markedly decreased compared with control group （ 100% ） （ P 〈 0. 05 ）. While Compared with model group, the survival rate of cells in the test - midst and test - high groups were 87.00% ,94. 12% with statistical difference （ P 〈 0. 05 ） . The activity of SOD was （ 23.56 ＋ 4. 19 ） U . mL-1 in model group. Compared with model group, the activity ofSOD was （32.98 ± 6. 13 ）U . mL-1 in test -high group with statistical difference （P 〈 0. 05 ）. The content of MDA was （3.96 ± 0. 79） nmol . mL-1 in model group. Compared with model group, the content of MDA were （ 2.98 ± 0. 59 ）, （2.81 ± 0. 63 ） nmol . mL- 1, in test - midst and test - high groups with statistical difference （ P 〈 0. 05 ）. And the leaking of LDH：Model group was （519. 67±103.78） U . L-1. Compared with model group, the leaking of LDH were （334. 58 ± 101.41 ）, （327.83± 99. 66）U. L-1 in testmidst and test- high groups with statistical difference （P 〈 0. 01 ）. Conclusion These results indicated that BCL possesses protective effects on 2O2 - injured hippocampal nerve cell in neonatal rats.