食管鳞状细胞癌NET1的表达和淋巴管浸润与病理的相关性

Relationship of NET1 expression and D2-40 labeled lymphatic vessel invasion with clinicopathologic features of esophageal squamous cell carcinoma

目的探讨食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)组织中神经上皮细胞转化基因1(neuroepithelial cell transforming gene 1,NET1)的表达及D2-40标记的肿瘤淋巴管浸润与病理之间的相关性。方法采用Western印迹法检测ESCC组织及正常食管组织中NET1蛋白的表达情况,免疫组织化学法检测ESCC中NET1及D2-40的表达情况,观察D2-40标记的淋巴管内癌细胞浸润(lymphatic vessel invasive,LVI)的情况,分析NET1、LVI与临床病理指标之间的关系及患者5年生存率的差异。结果 NET1在ESCC组织中阳性表达率(++/+++)为61.3%(20.4%/40.9%)(49/80),正常食管组织中表达阳性率为10.5%(2/19),2例均为弱阳性(+),LVI在ESCC组织中的阳性率为36.3%(29/80),NET1高表达、LVI阳性与ESCC的淋巴结阳性转移、临床分期呈正相关(P<0.05),与肿瘤的神经血管侵犯相关(P<0.05),而与性别、年龄、组织学分级无明显相关性(P>0.05)。NET1高表达与LVI阳性密切相关(P=0.012),NET1高表达或LVI阳性的ESCC患者术后的生存率明显降低,而且同时存在NET1高表达和LVI阳性的ESCC患者术后5年生存率最低。结论联合检测NET1及LVI可以提高对食管鳞癌患者的术后临床病理分期及预后的判断的准确性。

Objective To study the relationship of NET1 expression and D2-40 labeled lymphatic vessel invasion （LVI） with clinicopathological characteristics of esophageal squamous cel carcinoma （ESCC）. Methods The expression of NET1 in ESCC and normal esophageal tissue was detected by Western blot. Immunohistochemistry was used to detect the expression of NET1 and D2-40 protein in 80 cases of ESCC and D2-40 labeled LVI was identified. The relationship of NET1 expressions and D240 labeled LVI with clinical pathological characteristics of ESCC was analyzed. The overall 5-year survival rate was analyzed by Kaplan-Meier survival analysis. Results The positive expression（ ＋ ＋/＋ ＋ ＋ ） rate of NET1 was 61.3% （20.4%/40.9% ） in ESCC and 10.5% （ ＋ ） in normaJ esopnagea~ tissue. NET1 high expression and positive LVI was significantly correlated with positive lymph node metastasis, TNM stage, nerve and blood vessel invasion（P 〈 0.05 ）, but not with gender, age, tumor differentiation（P 〉 0. 05 ）. The positive correlation between NET1 high expression and positive LVI was found（ P = 0. 012 ）. The 5-year survival rate of patients with NET1 high expression or positive LVI was significantly lower. The 5-year survival rate of patients with NET1 high expression and positive LVI was lowest. Conclusion Combined detection of NET1 and D2-40 is of value in clinical staging and prognosis of esophageal squamous cell carcinoma.