植物精油复配抑菌性能分析

Compound Antibacterial Property of Plant Essential Oils

目的以对纸质品造成主要危害的黑曲霉、草本枝孢霉及球毛壳霉为实验菌株,研究牛至、柠檬草和丁香精油的复配抑菌性能。方法采用滤纸片扩散法研究3种植物精油的抑菌效果,并检测其抑菌持久性;通过三因子二次正交旋转设计探索3种植物精油两两复配的复合抑菌性能,并确定最优复配配方,利用气相色谱法对最优复配防霉剂的主要化学成分进行定性分析。结果牛至、柠檬草及丁香精油对黑曲霉的最低抑菌体积分数分别为0.05%,0.1%,0.2%,对草本枝孢霉的分别为0.05%,0.1%,0.2%,对球毛壳菌的分别为0.025%,0.05%,0.1%;牛至和柠檬草精油的最佳体积比为1∶1,其分级抑制体积分数的指数值为0.75,表现为相加作用。结论牛至与柠檬草精油复配后抑菌效果最为突出,且2种主要抑菌物质香芹酚和柠檬醛并未发生化学反应。

The work aims to research compound antibacterial properties of oregano, lemon grass and clove essential oil with aspergillus niger, herb cladosporium and chaetomium globosum as experimental strains. Aantimicrobial effect and antibacterial durability of three kinds of plant essential oils were researched by using filter paper diffusion method. The composite antibacterial properties of the compounds of any two among the three plant essential oils were explored by us- ing three factors quadratic orthogonal rotating design. The optimal compound formula was determined finally. Major chemical components of the optimal combination of fungicide were analyzed quantitatively. The results showed that： For aspergillus niger, the MIC of oregano, lemon grass and clove essential oils were 0.05%, 0.1%, and 0.2% respectively. For herb cladosporium, the MIC of oregano, lemon grass and clove essential oils were 0.05%, 0.1%, and 0.2% respectively. For chaetomium globosum, the MIC of oregano, lemon grass and clove essential oils were 0.025%, 0.05%, and 0.1% re- spectively. The best ratio of oregano and lemon grass essential oil was 1：1, and the FIC value was 0.75 which showed ad- ditive effect. In conclusion, antimicrobial effect of oregano and lemongrass essential oil compound was most prominent. The carvacrol and citral did not have chemical reaction.