乳腺癌组织中人乳头状瘤病毒与Rb、P53蛋白表达、预后的相关性分析

Correlation between human papilloma virus and expression of HPV,Rb and P53 and its prognosis in breast carcinoma

目的探讨人乳腺癌及乳腺良性病变组织中的人乳头状瘤病毒(HPV16/18)感染和Rb、P53蛋白表达与人乳腺癌发生发展及预后的关系。方法采用免疫组织化学SP法检测108例乳腺癌组织、54例乳腺纤维腺瘤及其周边正常乳腺组织HPV16/18,Rb、P53蛋白表达,分析其HPV16/18与Rb、P53蛋白表达、预后的相关性。结果 108例乳腺癌组织中HPV16/18、P53、Rb蛋白的阳性表达率分别为57.40%,37.03%,82.41%,均显著高于正常组及纤维腺瘤组(P<0.001),HPV16/18与P53蛋白的表达呈负相关(r=-0.503,P<0.001),而与Rb蛋白表达无相关性(P>0.05)。HPV16/18、P53阳性与阴性患者2年内的生存率差异有明显统计学意义(P<0.05),而Rb阳性与阴性患者2年内的生存率差异无明显统计学意义(P>0.05)。结论 HPV感染可能通过对P53蛋白的降解从而促进乳腺癌的发生、发展,并影响其预后,HPV感染可能是乳腺癌的高危因素之一。

Objective To investigate the correlation between human papilloma virus（ HPV16 and HPV18） infection and Rb,P53 protein expression and the prognosis in breast cancer. Methods SP immunohistochemistry was employed to detect the expression in 108 cases of breast cancer,54 cases of breast fibroadenoma and its corresponding adjacent normal breast tissues,and the correlations between HPV16 /18 and Rb and P53 expression,prognosis were analyzed. Results The positive expression rate of HPV16 /18,P53 and Rb in 108 cases of breast cancer was 57. 40%,37. 03% and 82. 41%,respectively,higher than those in normal breast tissues and breast fibroadenoma（ P〈0. 001）. The expression of HPV16 /18 was negatively correlated with the expression of P53（ r =- 0. 503,P〈0. 001）,but not correlated Rb protein expression（ P〉0. 05）. The 2-year survival rate was statistically significant between patients with positive and negative HPV16 /18,P53（ P〈0. 05）,but not statistically different between Rb positive and negative patients（ P〈0. 05）. Conclusion HPV16 /18 may promote the carcinogenesis of breast cancer by degradation of P53 protein. HPV16 /18 infection may be a high risk factor of breast cancer.