摘要
为探讨簇毛麦染色体对小麦品质的影响,利用A-PAGE、SDS-PAGE和高效毛细管电泳(High-performance capillary electrophoresis,HPCE)技术对12个易位系及7个附加系的贮藏蛋白进行鉴定。将簇毛麦的一条ω-醇溶蛋白基因定位于1VL。结果表明:易位系T1DL·1VS可用来改良小麦品质;定位于簇毛麦1VS染色体上的高分子谷蛋白在小麦中表达不稳定;其他材料醇溶蛋白组成发生改变较小,可能对面筋相关品质影响不大,在育种工作中不会引起面筋相关品质下降;易位系材料中的小麦醇溶蛋白的质量分数有所增加。
For studying the effects of Dasypyrum villosa chromsomes on grain processing quality of Chinese Spring（CS）, A-PAGE SDS-PAGE and HPCE were used to analyze to identify the seed storage protein of 12 translocation lines and 7 addition lines in this paper. One of the oo-Gliadin was located in the 1VL,a-Gliadin located in the 1VS,4VS and 6VL. The results showed that the translocation lines TIDL · 1VS could improve wheat quality. Expression level of the HMW-GS gene Glu-V1 of D. villo- sum which located on 1VS was low. The gliadin contents of other translocation lines changed a little, and it maybe had little effect on gluten quality, so it cannot decrease related gluten quality in wheat breeding, the gliadin content of wheat in translocation lines increased.
出处
《西北农业学报》
CAS
CSCD
北大核心
2016年第9期1304-1310,共7页
Acta Agriculturae Boreali-occidentalis Sinica
基金
"十二五"农村领域国家科技计划项目(2011AA100501)
国家现代农业产业技术体系建设专项(CARS-3-2-47)
国家自然科学基金(31171538
30900896)
中央高校基本科研业务费专项(QN2009007)~~
关键词
簇毛麦
易位系
贮藏蛋白
小麦品质
D. villosum
Translocation lines
Storage protein
Wheat quality
