RRLC法同时测定天王补心丸中6种木脂素类成分的含量

Simultaneous Determination of Six Lignans in Tianwang Buxin Pill by RRLC

目的:建立快速高分辩液相色谱(RRLC)同时测定天王补心丸中五味子醇甲、五味子醇乙、五味子酯甲、五味子甲素、五味子乙素、五味子丙素6种主要木脂素成分的方法。方法:采用TSK gel ODS-140HTP(50 mm×2.1 mm,2.3μm)色谱柱;流动相为乙腈-水梯度洗脱,流速0.3 m L·min-1;柱温30℃;检测波长250 nm,进样量10μL。结果:6种被测木脂素成分分离度良好;各成分质量与峰面积在测定范围内均呈良好的线性关系(r>0.9990),重复性良好;平均回收率分别为100.1%、99.3%、97.8%、99.1%、98.8%、97.5%,RSD分别为1.1%、1.8%、2.3%、2.5%、1.6%、2.1%。结论:此方法简便、准确、重复性好,经方法学验证可用于检测天王补心丸的质量。

Objective： To establish a RRLC method to determine schisandrin, schisandrol B, schisantherin A, deoxyschisandrin, schisandrin B and schisandrin C in Tianwang Buxin Pill. Methods： The TSK gel ODS-140HTP（50 mm×2.1 mm, 2.3 μm） was used. The mobile phase consisted of acetonitrile-water in gradient elution mode with a volume flow rate of 0.3 m L·min-1. The column temperature was set at 30 ℃. The detection wavelength was set at 250 nm. Results： Excellent chromatographic separation was achieved for six studied lignans with good linearity（r 0.9990） in the studied concentration ranges. The average recoveries were 100.1%, 99.3%, 97.8%, 99.1%, 98.8%, 97.5% and RSD were 1.1%, 1.8%, 2.3%, 2.5%, 1.6%, 2.1%, respectively. Conclusion： The method is efficient and accurate with a good reproducibility through the methodology validation, and can be used for the quality control of Tianwang Buxin Pill.