摘要
目的：建立利肝隆颗粒五味子中4种木脂素成分的含量测定方法。方法：采用HPLC法,色谱柱：十八烷基硅烷键合硅胶色谱柱,流动相：甲醇-水梯度洗脱,流速：1.0 m L·min-1,柱温：30℃,检测波长：230 nm。结果：被测的4种成分在各自浓度范围内线性关系良好,平均回收率为97.4%～99.6%,RSD为0.6%～0.8%。结论：本实验所建立的方法稳定可靠、简便可行,可用于该品种五味子木脂素类成分的定量分析。
Objective： To establish an HPLC method for determination of four lignans in Schisandrae chinensis fructus of Liganlong Granule. Methods： The HPLC method was carried out on a C18 column using gradient elution of methanol and water as the mobile phase, and the flow rate was 1.0 m L · min-1, the column temperature was 30 °C and the detection wavelength was 230 nm. Results： The calibration curves of four lignans all met the requirement. The average recovery was 97.4%-99.6%, and RSD was 0.6%-0.8%. Conclusion： This method is stable, convenient and reliable for quantitative analysis of lignans in Schisandrae chinensis fructus.
作者
肖宇
林林
张福明
张清波
笔雪艳
Xiao Yu Lin Lin Zhang Fuming Zhang Qingbo Bi Xueyan(Heilongjiang University of Chinese Medicine, Haerbin 150040, China Heilongjiang Institute for Food and Drug Control, Haerbin 150088, China Heilongjiang Food and Drug Administration, Haerbin 150001, China)
出处
《中国药事》
CAS
2016年第11期1117-1122,共6页
Chinese Pharmaceutical Affairs
关键词
五味子
五味子醇甲
五味子酯甲
五味子甲素
五味子乙素
利肝隆颗粒
Schisandrae chinensis fructus
schisandrin
schisantherin A
deoxyschisandrin
schisandrin B
Liganlong Granule
