红芸豆植物凝集素长期阴道给药对小鼠阴道上皮细胞的影响

The influence of vaginal injected red kidney bean lectin for a long time on vaginal epithelial cells of mice

目的:观察长期阴道使用红芸豆植物凝集素(RKBL)对小鼠阴道上皮细胞的影响。方法:使用不同浓度的RKBL注入小鼠阴道连续给药4个月后断颈处死小鼠,取其阴道及周围组织,制作石蜡切片并经苏木精染色,显微镜下观察阴道组织的形态学变化。结果:小鼠阴道在浓度≤2.5mg/只的RKBL处理后,无炎症病变,阴道粘膜上皮细胞形态完好无破损,皮下组织形态正常。而当给药浓度提高到5mg/只时,阴道黏膜浅层上皮细胞出现空泡化。结论:浓度≤2.5mg/只的RKBL长期使用不会引起阴道黏膜上皮细胞及皮下组织的不良反应。

Objective.. To explore the influence of vaginal injected red kidney bean lectin（RKBL） on vaginal epithelial cells of mice. Method;Mice were injected vagina of different concentrations of RKBL for four months continually, and all mice were decapitated after four months administration and their vagina and surrounding tissues were fixed in form- aldehyde solution. In order to investigating their histological changes, the vaginas tissues were done paraffin section and hematoxylin eosin stained. Result： When concentrations of RKBL at 2.5mg or below injected in each mouse for four months administration, vaginal mucous membrane were non hyperemia inflammation expression, and the vaginal epi- thelial ceils or tissues remained intact and normal form. While the concentration of RKBL increased to 5 mg, vaginal mucosa epithelial cells had changed with cavitation. Conclusion.. When the concentrations of RKBL at 2.5mg or below by vaginal injected for a long time, there is no any side effect on vaginal epithelial cells or subcutaneous tissue.