和颜坤泰胶囊对卵巢去势大鼠子宫形态及细胞凋亡的影响

Anti-apoptotic and uterotrophic effects of Heyan Kuntai capsules in uterus of ovariectomized rats

[目的]研究和颜坤泰胶囊对卵巢去势模型大鼠子宫形态及细胞凋亡的影响。[方法]以雌性去势大鼠为动物模型,分为空白对照组,假手术组,模型组,补佳乐组,莉芙敏组,和颜坤泰胶囊8、4、2 g生药/kg剂量组。给药8周后,观察药物对子宫病理学的影响,检测子宫细胞凋亡率及Bcl-2 m RNA、Bax m RNA和蛋白的表达。[结果]和颜坤泰胶囊可增加去势大鼠子宫横截面及壁厚,肌层面积及厚度,内膜面积及厚度,上皮厚度和腺体数(P<0.01,P<0.05);能降低去势大鼠子宫细胞的凋亡率(P<0.01,P<0.05),升高子宫Bcl-2 m RNA和蛋白的表达(P<0.01,P<0.05),降低Bax m RNA和蛋白的表达(P<0.01,P<0.05)。[结论]和颜坤泰胶囊有一定的子宫营养作用,可通过调控Bcl-2和Bax基因与蛋白的表达来抑制子宫细胞的凋亡。

[Objective] To investigate the effects of Heyan Kuntai capsules on uterus morphology and apoptosis in ovariectomized rats. [Methods] Female SD rats underwent ovariectomize surgery and were divided into model group, progynova group, Remifemin group and Heyan Kuntai capsule of high, medium and low dose groups （8, 4 and 2 g crude drug/kg respectively）. The drugs were administered for 8 weeks. This article observed the impacts of drugs on uterus pathology, staining and apoptosis, detected the expression levels of Bcl-2 and Bax mRNAs and proteins. [Results] It was obtained significantly decreased epithelial thickness and reduced myometrium and endometrium cross-sectional area and wall thickness in the model group compared with the control and Heyan Kuntai capsule treated groups（P〈0.01 or P〈0.05）. It was also decreased Bcl-2 and increased Bax mRNAs and proteins expressions （P〈O.O1 or P〈0.05） in the model group. Further more, the model group showed increased apoptosis rate （P〈0.01 or P〈0.05 ） and reduced number of glands （P〈0.01 or P〈0.05） in the uterus. [Conclusion] Heyan Kuntai capsules elicits uterotrophic effect and attenuates apoptosis in the uterus cells by regulating mRNAs and protein expressions of Bcl-2 and Bax.