基于Ussing Chamber技术的红景天苷大鼠肠黏膜透过性研究

Study on permeabilities of Salidroside via rat mesentery mucosa by using Ussing Chamber

[目的]研究红景天苷在大鼠离体肠黏膜中的透过情况。[方法]采用Ussing Chamber技术,考察不同浓度红景天苷于大鼠肠黏膜透过情况,并考察维拉帕米(Ver)和十二烷基硫酸钠(SDS)对红景天苷大鼠肠黏膜透过的影响。[结果]不同浓度红景天苷在大鼠不同肠段的单位时间转运速率(Flux)随浓度升高而增大,不同肠段表观渗透系数(P_(app))无统计学差异(P>0.05)。Ver加入一定量的SDS后,红景天苷在回肠和结肠的P_(app)显著增加(P<0.01或P<0.05),当SDS浓度为2 g/L时,回肠P_(app)增加为对照组的2.76倍。[结论]红景天苷在大鼠全肠段均有吸收,且吸收具有浓度依赖性,以被动吸收为主,Ver对红景天苷大鼠各肠吸收无影响,SDS促进了红景天苷回肠和结肠吸收。

[Objective] To investigate the permeability of salidroside in mesentery mucosa of rat ex vivo. [Methods] The Ussing Chamber was employed to investigate the ex vivo permeability in rat mesentery mucosa of salidroside with different concentrations or with the addition of verapamil and SDS. [Results] It was found that the Flux of salidroside was increased with the increasing concentration, and there was no difference（P〈0.05） in the apparent permeability coefficient （P~） in different mesentery segments of rat. The P^p of salidroside got significantly increased in ileum and colon （P〈0.01 or P〈0.05）, and reached to 2.76-fold compared with the control in ileum, when a certain amount of SDS was added in the donator side. [Conclusion] Salidroside could be slightly absorbed in all mesentery segments of rat with a passive concentration-dependent transportation. There were no effects of verapamil on permeation of salidroside, and SDS could significantly increase salidroside permeation in ileum and colon of rat.