兔脂肪肝内VX2移植瘤及炎性假瘤超声造影血流灌注及血管生成的对比研究

Comparative study on blood perfusion and angiogenesis of intrahepatic VX2 tumors and inflammato- ry pseudotumor by contrast-enhanced ultrasound in rabbits with fatty liver

目的对比分析脂肪肝内VX2移植瘤、炎性假瘤（IPL）超声造影（CEUS）血流灌注及血管生成的表达情况。方法建立兔脂肪肝内VX2移植瘤、IPL模型。应用QontraXt超声造影定量分析软件分析脂肪肝内vx2移植瘤、IPL血流灌注情况，免疫组化技术检测肿瘤微血管密度（MVD）及血管内皮生长因子（VEGF）表达水平。结果成功建立兔脂肪肝内VX2移植瘤及IPL结节各20个。兔脂肪肝内VX2移植瘤达峰时间、峰值强度、曲线下面积分别为（24．16±6．58）s、（52．29±13．24）dB、6．09±2．61；炎性假瘤分别为（29．86±6．75）s、（41．36±9．50）dB、3．86±1．00。兔脂肪肝内VX2移植瘤与IPL相比，造影剂达峰时间早，峰值强度及曲线下面积高，差异均有统计学意义（P〈0．05）。兔脂肪肝内VX2移植瘤MVD计数为45．21±8．80、VEGF表达为4．00±0．86；炎性假瘤分别为21．10±3．31、2．00±0．86。兔脂肪肝内VX2移植瘤与脂肪肝内IPL相比，MVD计数高，VEGF蛋白表达水平高，差异均有统计学意义（P〈0．05）。结论兔脂肪肝内VX2移植瘤CEUS灌注效应、MVD计数及VEGF蛋白表达水平均较IPL高。低机械指数超声造影结合QontraXt定量分析，可准确反映脂肪肝背景下良恶性病变的血流灌注情况及恶性肿瘤的血管生成。

Objective To analyze contrast-enhanced ultrasound （CEUS） evaluation on blood perfusion of intrahepatic VX2 tumors and inflammatory pseudotumor in rabbits with fatty liver and further compare the angiogenesis expression. Methods Intrahepatic VX2 tumors and inflammatory pseudotumor in rabbit with fatty liver models were well established. The blood perfusion of VX2 tumors and inflammatory pseudotu- mor in rabbit fatty liver was analyzed using QontraXt CEUS quantitative analysis software, and the microvascular density （MVD） and vascular endothelial growth factor （VEGF） expression were detected by immunohistochemistry, respectively. Results Twenty VX2 tumor modds and 20 inflammatory pseudotumor in fatty liver were successfully established in rabbits. The time to peak （TP）, peak intensity （PI） and the area under the curve （AUC） of VX2 tumor in rabbits with fat liver were （24.16 ± 6.58） s, （52.29 ± 13.24） dB, 6.09 ± 2.61 ; The TP, PI and AUC of IPL in fatty liver were （29.86 ~ 6.75） s, （41.36 _＋ 9.50） dB, 3.86± 1.00, respectively. Compared with those for inflammatory pseudotumor lesions in fatty liver rabbits, the time to peak for VX2 tumor lesions were earlier, peak intensity was higher, and area under the curve was larger （P 〈 0.05 ）. The MVD count of VX2 tumor in rabbits with fatty liver was 45.21 ±8.80, VEGF expression of them was 4.00 ± 0.86 ; MVD count and VEGF expression of IPL in fatty livers was 21.10 ± 3.31, 2.00 ± 0.86, respectively. Compared with those for inflammatory pseudotumor lesions in fatty liver rabbits, MVD count and VEGF protein expression for VX2 tumors were significantly higher （ P 〈 0. 05 ）.Conclusions The CEUS blood perfusion, MVD count, and VEGF protein expression for VX2 tumor lesions were higher than those for inflammatory pseudotumor lesions in rabbits with fatty liver. Low mechanical index contrast-enhanced ultrasound combined with QontraXt quantitative analysis software can accurately reflect blood perfusion of benign and malignant tumor as well as angiogenesis of malignancies in fatty liver.