摘要
对分离自云南大学校园的攀枝花苏铁根际土壤的链霉菌Streptomyces albiflaviniger菌株YN-10-2的化学成分进行结构和活性的研究.从20L固体表面发酵产物的提取物中经过多种层析方法的综合运用,共分离纯化出6个纳皮拉霉素(napyradiomycin)家族化合物.通过对核磁共振谱图和质谱数据的解析以及文献的对照,确定了化合物的化学结构,分别为napyradiomycin B1(1)、napyradiomycin B3(2)、napyradiomycin C1(3)、napyradiomycin A2a(4)、napyradiomycin A2b(5)和3-chloro-6,8-dihydroxy-8-a-lapachone(6),其中化合物4和5是差向异构体.在滤纸片抗菌实验中,化合物1~3显示出对金黄色葡萄球菌的抑制活性.
A rhizosphere strain Streptomyces albiflaviniger YN-10-2 isolated from Cycas panzhihuaensis Zhou L et Yang S Y at Yunnan University was chosen to study its secondary metabolites.Six napyradiomycins compounds were purified from 20 L solid fer-mentation culture.Their chemical structures were elucidated mainly by spectroscopic analyses including nuclear magnetic resonance (NMR)spectroscopy and electrospray ionization mass spectroscopy(ESI-MS).Results showed that these six compounds were identi-fied as napyradiomycin B1(1),napyradiomycin B3(2),napyradiomycin C1(3),napyradiomycin A2a(4),napyradiomycin A2b(5)and 3-chloro-6,8-dihydroxy-8-α-lapachone(6),respectively.Among these compounds,napyradiomycins A2a(4)and A2b(5)are epimers. Additionally,compounds 1-3 exhibited inhibitory activities against Staphylococcus aureus in the antimicrobial tests by disc diffusion assay.
出处
《厦门大学学报(自然科学版)》
CAS
CSCD
北大核心
2016年第4期478-484,共7页
Journal of Xiamen University:Natural Science
基金
厦门市科技计划项目(3502z20123010)