来源于链霉菌Streptomyces albiflaviniger菌株YN-10-2的纳皮拉霉素类化合物的结构和活性

Structures and Bioactivities of Napyradiomycins Compounds from Streptomyces albiflaviniger YN-10-2

对分离自云南大学校园的攀枝花苏铁根际土壤的链霉菌Streptomyces albiflaviniger菌株YN-10-2的化学成分进行结构和活性的研究.从20L固体表面发酵产物的提取物中经过多种层析方法的综合运用,共分离纯化出6个纳皮拉霉素（napyradiomycin）家族化合物.通过对核磁共振谱图和质谱数据的解析以及文献的对照,确定了化合物的化学结构,分别为napyradiomycin B1（1）、napyradiomycin B3（2）、napyradiomycin C1（3）、napyradiomycin A2a（4）、napyradiomycin A2b（5）和3-chloro-6,8-dihydroxy-8-a-lapachone（6）,其中化合物4和5是差向异构体.在滤纸片抗菌实验中,化合物1~3显示出对金黄色葡萄球菌的抑制活性.

A rhizosphere strain Streptomyces albiflaviniger YN-10-2 isolated from Cycas panzhihuaensis Zhou L et Yang S Y at Yunnan University was chosen to study its secondary metabolites.Six napyradiomycins compounds were purified from 20 L solid fer-mentation culture.Their chemical structures were elucidated mainly by spectroscopic analyses including nuclear magnetic resonance （NMR）spectroscopy and electrospray ionization mass spectroscopy（ESI-MS）.Results showed that these six compounds were identi-fied as napyradiomycin B1（1）,napyradiomycin B3（2）,napyradiomycin C1（3）,napyradiomycin A2a（4）,napyradiomycin A2b（5）and 3-chloro-6,8-dihydroxy-8-α-lapachone（6）,respectively.Among these compounds,napyradiomycins A2a（4）and A2b（5）are epimers. Additionally,compounds 1-3 exhibited inhibitory activities against Staphylococcus aureus in the antimicrobial tests by disc diffusion assay.