色胺酮在食管癌化疗耐药过程中的作用

Effect of Tryptanthrin on Drug Resistance During Chemotherapy of Esophageal Cancer

为研究色胺酮对食管癌化疗药物顺铂耐药性的抑制作用及其作用机制,设计食管癌Eca109细胞及其顺铂耐药株Eca109/cDDP细胞的顺铂及色胺酮不同处理组,根据实验目的选择不同的处理方式.利用实时荧光定量PCR检测Eca109和Eca109/cDDP细胞中多药耐药基因1(MDR1)及谷胱甘肽-巯基-转移酶-pi基因(GST-pi)的mRNA表达水平;免疫印迹和免疫荧光技术检测Eca109细胞和Eca109/cDDP细胞中MDR1和GST-pi蛋白的表达水平;细胞计数试剂盒(cell counting kit,CCK-8)法检测细胞增殖以及基因敲降后的回补效果.结果表明:色胺酮不仅显著抑制了食管癌细胞顺铂耐药株Eca109/cDDP细胞中MDR1基因的表达,而且MDR1和GST-pi蛋白的表达也受到了显著的抑制;色胺酮不但能抑制Eca109细胞的增殖能力,对耐药株Eca109/cDDP细胞增殖也存在抑制作用;色胺酮逆转耐药性的能力依赖于对MDR1和GST-pi的表达抑制.综上分析,色胺酮能够通过抑制MDR1和GST-pi蛋白的表达来逆转食管癌细胞顺铂耐药株的耐药性,是一种潜在的化疗辅助药物.

To study the inhibitory effect of tryptanthrin on drug resistance to cisplatin chemotherapy for esophageal carcinoma and its mechanism,different treatments （blank control,cisplatin treatment,tryptanthrin treatment and cisplatin combined tryptanthrin treatment）of esophageal cancer Eca109 cells and cisplatin-resistant Eca109 cells （Eca109/cDDP）were performed on the basis of the purpose of experiments.Real-time quantitative PCR was used to detect the mRNA levels of multidrug resistance gene 1（MDR1）and glutathione-s-transferase-pi gene （GST-pi）in Eca109 and Eca109/cDDP cells.Western blot and/or immunofluorescence were used to detect the protein levels of MDR1 and GST-pi.The proliferation of cells was tested by cell counting kit （CCK-8）.The results showed that tryptanthrin suppressed cell proliferation by inhibition of mRNA and protein levels of MDR1 and GST-pi in Eca109/cDDP cells. In summary,tryptanthrin can reverse the cisplatin resistance in Eca109/cDDP by its inhibition on MDR1 and GST-pi expression.It could be a potential adj uvant agent for chemotherapy.