摘要
目的探讨氯乙烯(VCM)染毒对雄性大鼠肝组织及血清中mir-122表达量的影响。方法将无特定病原体级雄性SD大鼠分为对照组和低、中、高剂量组,每组24只。腹腔注射剂量分别为0、5、25和125 mg/kg体质量的VCM气体染毒,每周3次(隔日染毒)。在染毒第6、8和12周末,每组随机处死8只大鼠,分别提取肝组织中的小片段RNA(<200 nt)和血清中总RNA,采用实时荧光定量聚合酶链式反应检测mir-122相对表达水平。结果分别与同时间点对照组和低剂量组比较,中和高剂量组大鼠肝组织中mir-122相对表达水平在染毒第6周均上调(P<0.05),在染毒第12周均下调(P<0.05);中和高剂量组大鼠肝组织中mir-122相对表达水平均呈随时间增加而下降的趋势(P<0.05)。与同时间点对照组比较,3个剂量组大鼠血清中mir-122相对表达水平在染毒第8周均上调(P<0.05),在染毒第12周均下调(P<0.05);3个剂量组大鼠血清中mir-122相对表达水平在第12周均低于同组第6和8周(P<0.05)。结论 VCM可导致大鼠肝组织及血清中mir-122表达的改变;mir-122可能是VCM暴露所致的效应标志物之一。
Objective To explore the effects of vinyl chloride monomer（ VCM） on the expression of mir-122 in the liver tissue and serum of rat. Methods Specific pathogen free adult male SD rats were randomly divided into 4 groups with 24 rats in each group： the control group,the low-,medium- and high-dose groups. The 4 groups were intraperitoneally injected with VCM exposure levels at 0,5,25 and 125 mg / kg body weight 3 times per week,respectively. Eight rats per group were randomly selected and sacrificed after 6,8 and 12 weeks. Small RNA（ 〈200 nt） in the liver tissue and total RNA in the serum were isolated and purified. Real-time fluorescent quantitative polymease chain reaction technique was used to detect their expressions of mir-122. Results Compared with the control group and the low-dose group at the same time points,the relative expressions of mir-122 in the liver tissue of rats in the medium- and high-dose groups were significantly increased after exposed to VCM for 6 weeks（ P〈0. 05）,and then were significantly decreased after exposed to VCM for 12 weeks（ P〈0. 05）. The relative expressions of mir-122 in the liver tissue of rats in the medium- and highdose groups were decreased with VCM exposure time（ P〈0. 05）. Compared with the control group at the same time points,the relative expressions of mir-122 in serum of rats in the three dose groups were significantly increased after being exposed to VCM for 8 weeks（ P〈0. 05）,and then were significantly decreased after being exposed to VCM for 12 weeks（P〈0. 05）. Among the three dose groups,the relative expressions of mir-122 in serum of rats after 12-week exposure were lower than those after 6-week and 8-week exposure in the same group respectively（ P〈0. 05）. Conclusions The VCM exposure significantly changes the expression of mir-122 in the liver tissue and serum of rat,which suggested that mir-122 may be one of the effect biomarkers for VCM exposure.
出处
《中国职业医学》
CAS
北大核心
2016年第5期542-546,共5页
China Occupational Medicine
基金
山西省基础研究计划项目(20130110591-1)
