白介素-17A对恢复期病毒性心肌炎小鼠抗ANT抗体的影响

THE EFFECT OF IL-17A ON ANTI-ADENINE NUCLEOTIDE TRANSLOCATOR(ANT)AUTOANTIBODIES IN MICE WITH VIRAL MYOCARDITIS AT RECOVERY STAGES

目的:观察白介素-17A(IL-17A)对恢复期病毒性心肌炎小鼠血清抗线粒体内膜ADP/ATP载体自身抗体(抗ANT抗体)水平的影响。方法:IL-17A敲除型(IL-17A-/-)以及野生型(WT)Balb/c小鼠腹腔注射含柯萨奇病毒B3(CVB3)的磷酸盐缓冲溶液(PBS)建立病毒性心肌炎小鼠模型(VMC-IL-17A-/-组和VMC-WT组),WT Balb/c小鼠腹腔注射PBS作为健康-WT组,注射病毒液或PBS后第42天留取血清,ELISA法测定血清中抗ANT抗体水平;取心肌组织作石蜡切片,HE染色观察心肌组织病理改变。结果:与健康-WT组相比,VMC-WT组小鼠血清中抗ANT抗体水平显著升高,差异有统计学意义(P<0.05);而与VMC-WT组相比,VMC-IL-17A-/-组小鼠血清中抗ANT抗体水平明显降低,差异有统计学意义(P<0.05)。结论:IL-17A促进恢复期VMC小鼠血清抗ANT抗体的产生。

Objective：To explore the effect of interleukins-17A（IL-17A）on the level of anti-adenine nucleotide translocator（ANT）autoantibodies in mice with viral myocarditis（VMC）at the recovery stage.Methods：Male wild-type（WT）and IL-17A-deficient（IL-17A-/-）Balb/c mice were intraperitoneally（i.p）injected with coxsackie virus B3（CVB3）to establish VMC models（VMC-WT group and VMC-IL-17A-/-group）.Meanwhile,a control group（WT group）of WT mice were established and treated with phosphate buffered saline i.p.Paraffin sections of cardiac tissues were prepared 42 days after CVB3 injection.Myocardial histopathologic changes were evaluated by hematoxylin-eosin staining.The level of anti-ANT autoantibodies was measured by enzyme-linked immunosorbent assay（ELISA）.Results：Compared with WT group,the level of anti-ANT autoantibodies was significantly increased in VMC-WT group（P〈0.05）.Compared with VMC-WT group,the level of anti-ANT autoantibodies was reduced in VMC-IL-17A-/-group（P〈0.05）.Conclusion：IL-17 Acontributes to the secretion of anti-ANT autoantibodies of VMC mice at the recovery stage.