相思藤水提物对小鼠化学性及酒精性肝损伤保护作用的研究

STUDY ON PROTECTIVE EFFECT OF XIANGSITENG AQUEOUS EXTRACT AGAINST ACUTE CHEMICAL AND ALCOHOLIC LIVER INJURIES IN MICE

目的:研究相思藤水提物(XSTE)对小鼠化学性及酒精性肝损伤的保护作用及机制。方法:分别采用四氯化碳(CCl4)、对乙酰氨基酚(AP)腹腔注射小鼠,建立化学性肝损伤模型;用50%酒精灌胃小鼠建立酒精性肝损伤模型。将昆明种小鼠随机分为正常对照组、肝损伤模型组、联苯双酯(BPDC)阳性对照组、XSTE高、中、低剂量组。计算肝脏重量指数和脾脏重量指数,测定血清谷草转氨酶(AST)、谷丙转氨酶(ALT)活性,肝组织丙二醛(MDA)和谷胱甘肽(GSH)含量、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性,酒精性肝损伤研究测定血清乙醇浓度、抗利尿激素(ADH)和乙醛脱氢酶(ALDH)。结果:与模型组比较,XSTE能减轻肝脏和脾脏重量,降低血清AST、ALT活性,降低血中乙醇浓度,增加ADH和ALDH活性,降低肝组织MDA含量,增加肝组织SOD、GSH-Px活性,增加GSH含量(P<0.05或P<0.01)。结论:XSTE对小鼠化学性及酒精性肝损伤具有明显的保护作用,其机制可能与其抗氧自由基、抑制脂质过氧化作用有关。

Objective：To investigate the effect and mechanism of Xiang Si Teng aqueous extract（XSTE）on CCl4-,acetaminophen（AP）-and alcoholic-induced acute liver injuries in mice.Methods：The mice were given by gavage with 50%ethanol,and by intraperitoneal injection with CCl4 or AP to establish models of alcoholic liver injury and chemical liver injuries,respectively.The mice were randomized into 6groups：normal control,model control,positive control,low-,medium-and high-dose XSTE-treated groups.The liver and spleen indexes were calculated.The activities of ALT and AST in the serum,and the levels of SOD,MDA,GSH-Px and GSH in liver tissue were measured.The alcohol concentration and the activities of ADH and ALDH were also detected in the study of alcoholic liver injury.Results：Compared with the model group,XSTE significantly reduced indexes of liver and spleen as well as the levels of MDA,AST and ALT,while increased the levels of SOD,GSH and GSH-Px significantly（P〈0.05 or P〈0.01）.It also reduced the alcohol concentration and the activities of ADH and ALDH.Conclusion：XSTE could protect the mice from CCl4-and AP-induced acute chemical liver injuries and attenuate alcoholic liver injury,and its mechanisms may be related to its effect in attenuating free radical and inhibiting the lipid peroxidation.