高山被孢霉产花生四烯酸的发酵和老化工艺

Fermentation and Cell Aging Strategy for Arachidonic Acid Production by Mortierella alpina

高山被孢霉对于淀粉质原料具有优良的利用能力。采用经淀粉酶液化的玉米粉-豆粕培养基,研究了高山被孢霉在5 L容积机械搅拌罐液态发酵的主要参数,如搅拌转速和通气体积比对于高山被孢霉发酵产花生四烯酸的影响,得到最优的搅拌转速和通气体积比,分别为200 r/min和1∶1。研究发现,采用发酵罐培养5 d的菌丝体,最佳的老化温度和保温时间分别是20℃和5 d。本研究把谷物粗原料成功用于高山被孢霉发酵产花生四烯酸,经发酵罐优化和菌体老化后的花生四烯酸产量达到11.7 g/L,菌体老化可以在保持产量的同时缩短发酵周期,有效降低生产成本,有望实际用于花生四烯酸的工业化生产。

Mortierella alpina possesses the excellent ability of using starch-based materials to produce arachidonic acid, while the production need to be verified in a fermentor. This study focused on fermentation and cell aging strategy to reduce production cost. Starch liquefaction was applied to improve media rheologyand dissolved oxygen.The results indicated that the best mixing speed and ventilation was 200 r/min and 1 ：1,respectively. The study also found that the optimal temperature and aging time was 20 ℃ and 5 d respectively. Cell aging can effectively reduce the production cost while maintaining production. Arachidonic acid production reached 11.7 g/L when the optimal fermentation and cell aging strategy was employed which allowed large-scale production of arachidonic acid by using raw crop meals.