箬叶中4种黄酮苷的同步HPLC检测

Simultaneous Determination of 4 Flavonoid Glycosides in Indocalmus Leaf by HPLC

箬叶中含有丰富的黄酮类化合物,具有多种生物活性。采摘国内箬叶主产区浙江松阳、武义,以及安徽黄山、福建光泽、江西宁都的样品。样品经体积分数50%乙醇提取,随后利用高效液相色谱法(HPLC)快速检测其中异荭草素、荭草素、牡荆素、异牡荆素含量,方法简单准确。结果显示,不同产地的箬叶均含有较丰富的黄酮苷。该结果揭示了国内不同产地的箬叶中异荭草素、荭草素、牡荆素、异牡荆素的含量差异,为全面深入研发箬叶提供采收情况和质量评价参考。

lndocalmus leaf is rich in flavonoids,which have various kinds of biological activities. Indocalmus leaves were collected from Songyang （Zhejiang province）, Wuyi （Zhejiang province）, Huangshan （Anhui province）,Guangze （Fujian province） and Ningdu （Jiangxi province）. Samples were extracted by 50% ethanol and flavonoids including isoorientin,orientin,vitexin and isovitexin were then determined by high performance liquid chromatography （HPLC）. Results indicated the richness of favonoids in samples and the difference among isoorientin,orientin,vitexin and isovitexin in lndocalmus leaves from different regions,and thus provided a reference for deep research and development of Indocolmus leaf.