山药多糖促进小鼠巨噬细胞向M1型极化

Rhizoma dioscoreae polysaccharide promotes M1 macrophage polarization in mice

目的考察山药多糖对小鼠巨噬细胞极化的影响。方法在体外实验中,将RAW264.7细胞与山药多糖共孵育24 h,采用MTT法检测细胞活性;利用Griess试剂盒检测NO分泌水平;采用CBA法检测细胞因子分泌水平;采用流式细胞术检测细胞表面MHC-Ⅱ类分子表达水平。在动物实验中,将Balb/c小鼠随机分为正常组、模型组、山药多糖低剂量组（50 mg/kg）和高剂量组（200 mg/kg）,每天灌胃给药1次连续15 d;第11~15天除正常组外,其余小鼠腹腔注射氢化可的松。末次给药第2天取脾和胸腺称质量,计算免疫器官指数;利用流式细胞术检测腹腔巨噬细胞MHC-Ⅱ类分子表达水平。结果山药多糖剂量低于500μg/ml时对RAW264.7细胞活性无显著影响,但可以促进细胞分泌NO和细胞因子IL-6、TNF-α、MCP-1,提高细胞表达MHC-Ⅱ类分子的水平。动物实验结果显示山药多糖可以恢复免疫抑制小鼠腹腔巨噬细胞MHC-Ⅱ类分子表达水平。结论研究表明山药多糖可以促进巨噬细胞向M1型极化。

This study was designed to explore the effects of Rhizoma dioscoreae polysaccharide（RDPS） onmacrophage polarization in mice. RAW264.7 cells were treated with RDPS for 24 h in vitro. Cell viability wasmeasured by MTT assay; NO content in cell supernatant was measured with Griess reagent; cytokine content incell supernatant was measured with CBA kit; the expression of MHC class Ⅱ molecule on cell surface wasanalyzed by flow cytometry. Balb/c mice were randomly divided into control group, model group, RDPS low-dosegroup（50 mg/kg） and high-dose group（200 mg/kg）. Mice were administrated intragastricly with RDPS orphysiological saline once a day for 15 consecutive days. On day 11 to day15, mice in model group and RDPS treatedgroups were injected intraperitoneally with hydrocortisone sodium succinate injection（50 mg/kg） once a day for 5consecutive days, while the control mice were given an equal volume of sterile physiological saline. On day 16,spleen indexes and thymus indexes were measured. The in vitro results showed that RDPS had no significant effecton cell viability of RAW264.7 cells at dose of less than 500 μg/ml. RDPS could significantly promote the secretionof NO, IL-6, TNF-α, MCP-1 and the expression of MHC class Ⅱ molecules on RAW264.7 cells. The in vivo resultsshowed that RDPS promoted the recovery of MHC class Ⅱ molecules expression on peritoneal macrophage. Theseresults demonstrate that RDPS can promote M1 polarization of mouse macrophage.