酿酒葡萄皮渣对单栏饲养方式下公绵羊繁殖性能及睾丸抗氧化性的影响

Effect of Dietary Wine Grape Pomace on Reproductive Performance,Antioxidant Ability of Testis in Ram

旨在研究日粮中添加一定量的酿酒葡萄皮渣对绵羊繁殖性能及睾丸抗氧化性能的影响。试验选取30只5月龄,体重(25±1)kg的杜泊×小尾寒羊杂交公羊,采用完全随机设计平均分为5组。1组自由活动,其余4组单栏饲养,建立应激模型。自由活动绵羊饲喂未添加葡萄皮渣日粮,单栏饲养绵羊分别饲喂不同葡萄皮渣添加水平(0%、5%、10%、20%)日粮。试验期80d。试验结束后,屠宰取样,测定睾丸系数并作附睾精子分析,测定睾丸组织抗氧化水平,对睾丸组织中Cu-ZnSOD、CAT、GPx4及Nrf2mRNA和蛋白相对表达量进行研究。结果表明,与自由活动组相比,单栏组绵羊睾丸组织中MDA及ROS水平升高(P<0.01),睾丸重量显著降低(P<0.05),睾丸系数也表现出下降趋势(P=0.06),附睾精子密度及顶体完整率显著降低(P<0.05),精子活动率极显著降低(P<0.01),精子畸形率极显著升高(P<0.01),表明单栏饲养方式对绵羊造成氧化应激,并在一定程度上影响绵羊繁殖性能。适量添加酿酒葡萄皮渣后,绵羊附睾精子密度和精子活动率均显著升高(P<0.05),精子畸形率显著降低(P<0.05),睾丸组织MDA含量显著下降(P<0.01),SOD、CAT及GSH-Px活性均显著提高(P<0.01,P<0.05,P<0.01);睾丸组织中Cu-ZnSOD mRNA相对表达量显著提高(P<0.05),Cu-ZnSOD、CAT、GPx4蛋白相对表达量显著提高(P<0.05)。综上表明,酿酒葡萄皮渣可以提高单栏饲养方式下绵羊睾丸组织抗氧化性,进而改善绵羊繁殖性能。

This experiment was conducted to investigate the effect of wine grape pomace supplementation to base diet on reproductive performance and antioxidant ability of testis in ram.A total of thirty 5-month-old with average weight of（25±1）kg Dorper（♂）× Small Tail Han sheep（♀）F1 male lambs were randomly selected and divided into 5groups equally.To establish a stress model,lambs in one of groups were raised freely,while the other 4groups were confined in stall.Lambs raised freely were fed with a base diet without grape pomace,and other lambs were fed with diets supplemented with grape pomace at 0%,5%,10%,20%,respectively.The experiment lasted for 80 d.At the end of experiment,all lambs were slaughtered and sampled.Testis coefficient,epididymal sperm indicators,antioxidant levels of testis were analyzed,and moreover,expression of Cu/ZnSOD,CAT,GPx4,Nrf2 mRNA and protein in testis were detected.The results showed that,compared with free group,MDA and ROS level of testis significantly increased（P〈0.01）,testis weight of lambs significantly decreased（P〈0.05）,and the organ coefficient of testis had a trend to be lower（P=0.06）in confined group with 0% wine grape pomace;epididymal sperm density,acrosomal integrity and motility were lower（P〈0.05 and P〈0.01,respectively）,while epididymal sperm deformity higher（P〈0.01）,in confined group with 0% wine grape pomace than those in free group.These results showed that ram raised in confined condition could cause oxidative stress and affect the reproductive performance.However,compared with confined group with 0% wine grape pomace,epididymal sperm density and motility were higher,while deformity lower,in wine grape pomace-added groups（P〈0.05）.MDA level of testis significantly decreased（P〈0.01）,but SOD,CAT and GSH-Px activity significantly increased（P〈0.01,P〈0.05 and P〈0.01）in wine grape pomace-added groups.Furthermore,the mRNA expression of Cu-ZnSOD and protein expression of Cu-ZnSOD,GPx4 and CAT in testis significantly increased（P〈0.05）in wine grape pomace-added groups.The results indicate that wine grape pomace can enhance antioxidant capacity in testis and improve the reproductive performance of separated-raising lambs.