CCND1基因G870A多态性与消化系统肿瘤的相关性

Correlation analysis of G870A CCND1 gene polymorphism with digestive system tumors

目的探讨CCND1基因G870A多态性与消化系统肿瘤的相关性。方法选取我院2010年8月-2014年8月的164例消化系统肿瘤患者作为病例组（分为胃癌组、结直肠癌组各82例）,选取同期健康体检的82例健康人群作为对照组,并采用聚合酶链反应一限制性片段长度多态性（PCR-RFLP）方法对3组研究对象的CCND1基因G870A基因分布频率分析,并与消化系统肿瘤发病的分期、分级的关系进行分析。结果胃癌组、结直肠癌组的CCND1基因G870A位点中的GG、GA和AA的分布频率与对照组存在明显差异（P〈0.05）;CCND1基因G870A位点多态性与消化系统肿瘤发病的危险升高具有良好的相关性（P〈0.05）;GA和AA基因型发生消化系统肿瘤发病危险明显高于GG基因型,具有统计学意义（P〈0.05）;而且高分化和高分期患者的基因型分布率高于低分化和低分期的患者,具有统计学意义（P〈0.05）。结论 CCND1基因G870A多态性与消化系统肿瘤发病危险有关,而且含有A基因型更容易发生消化系统肿瘤,与消化系统肿瘤分化和分期相关。

Objective To study the correlation of G870 A CCND1 gene polymorphism and digestive system tumors. Methods From August 2010 to August 2014, 164 digestive system cancer patients（including 82 patients with gastric cancer and 82 with colorectal cancer） and 82 healthy subjects（control group） were examined with PCR-restriction fragment length polymorphism（PCR-RFLP）. The distribution of CCND1 gene G870 A frequency in the 3 groups and its association with tumor staging and grading were analyzed. Results The frequencies of the GG, GA and AA genotypes in G870 A CCND1 gene loci in patients with gastric cancer and colorectal cancer differed significantly from those in the control group（P〈0.05）. G870 A CCND1 gene polymorphism was closely associated with an increased risk of digestive system tumors（P〈0.05）. The GA and AA genotypes were associated with a significantly higher risk of digestive system cancer risk than the GG genotype（P〈0.05）, and their frequencies were significantly higher in patients with tumors of higher pathological grade and in those in advanced tumor stages（P〈0.05）. Conclusion G870 A CCND1 gene polymorphism is associated with the risk of digestive system tumors. The allele A is associated with an increased risk of digestive system tumors and correlated with the tumor differentiation and staging of the tumor.