白藜芦醇对秀丽隐杆线虫寿命的影响及其初步机制研究

Effect of Resveratrol on Longevity of Caenorhabditis Elegans and Its Potential Mechanism

目的:探讨不同浓度白藜芦醇对秀丽隐杆线虫寿命的影响及其机制。方法:观察25、50、100μmol·L^(-1)白藜芦醇处理的秀丽隐杆线虫野生型N2与daf-2、daf-16突变体的寿命变化。结果:与对照组比较,25、50、100μmol·L^(-1)自藜芦醇处理均可以显著延长三种秀丽隐杆线虫的寿命。结论:白藜芦醇可以显著延长秀丽隐杆线虫的寿命,其对寿命的影响不依赖于DAF-2/胰岛素样信号传导通路。

Objective：To investigate the effect of resveratrol on longevity of Caenorhabditis elegans（C.elegans） and its potential mechanism.Methods：Three types of C.elegans,wild type（N2）,daf-2 mutant（el370） and daf-16 mutant（mu86） were cultured with different concentrations of resveratrol（（0、25、50、100μmol·L^（-1））.The survival rates were measured and the effect of resveratrol on life span was recorded.Results：Compared with the DMSO group,significantly extension of the normal life span of three types of C.elegans（N2,daf-2 and daf-16 mutants） was observed containing 25、50、100μmol·L^（-1） resveratrol.Conclusion：Resveratrol can extend the life span of C.elegans,possible not associated with DAF-2/insulin-like signaling pathway.