隔山香药材中异莳萝脑乙二醇的薄层鉴别及含量测定

Identification and Content Determination of Isodillapiolglycol in Radix Osterici Citriodori by TLC and HPLC

目的建立隔山香药材中异莳萝脑乙二醇的TLC鉴别及HPLC含量测定方法。方法采用TLC法,以三氯甲烷-丙酮（5∶1）为展开剂,10%硫酸乙醇溶液为显色剂,对隔山香药材中的异莳萝脑乙二醇进行鉴别。采用HPLC法测定隔山香药材中异莳萝脑乙二醇的含量,色谱柱：Diamonsil C18色谱柱（5μm,250 mm×4.6 mm）;流动相为甲醇-水（32∶68）;流速为1.0 m L·min-1;检测波长为210 nm;柱温为25℃;进样量：10μL。结果在优化色谱条件下薄层斑点清晰,分离效果好,方法耐用性佳;异莳萝脑乙二醇在0.0468~0.468μg范围内线性关系良好（r=0.9999）,平均回收率为101.4%,RSD为1.4%。结论本研究所建立的定性定量方法简便、准确、重现性好,可用于隔山香药材的质量控制。

Objective To establish methods for the identification and determination of isodillapiolglycol in Radix Osterici Citriodori（Geshanxiang）.Methods Thin layer chromatography（TLC） was performed using chloroformacetone（5∶1）as developing solvent and 10 % sulfuric acid ethanol solution as colorimetric agent.High performance liquid chromatography（HPLC）was performed on Diamonsil C_（18）column（5 μm,250 mm×4.6 mm）with the mobile phase of methanol-water（32∶68）.The flow rate was 1.0 m L·min^-1and the column temperature was kept at 25 ℃.The detective wavelength was set at 210 nm and the injection volume was 10 μL.Results The TLC spots were clear and can be well separated under the optimal chromatographic conditions.The calibration curve of isodillapiolglycol showed a good linearity in the range of 0.0468-0.468 μg（r=0.9999）.The average recovery was 101.4 % and RSD was 1.4 %（n=6）.Conclusion The methods are simple,accurate and reproducible,and can be used for the quality control of Radix Osterici Citriodori.