变频振动对尾吊大鼠失重性骨质疏松防治作用的研究

The therapeutic effect of frequency vibration on osteoporosis in tail-suspension SD rats subjected to simulated weightlessness

目的探讨变频振动对尾吊大鼠失重性骨质疏松模型的防治作用。方法采用目前世界上广泛应用且技术已经成熟的鼠尾悬吊动物模型模拟失重环境,应用电磁振动台将振动强度为0.5 g（g为加速度）在不同频率45 Hz、90 Hz、变频（5-90Hz）振动应力作用于3月龄SD雄性大鼠40只,随机分成5组,每组8只：对照组（非鼠尾悬吊组）;45 Hz组、90 Hz组、变频（5-90 Hz）组,鼠尾悬吊非振动组,每次振动10 min,每天1次,每周5次,休息间隔为2天,共4周。所有对照组不进行振动治疗,但置于与振动治疗组相同噪音环境下。实验后用Micro-CT测量股骨远端骨量、自动化分析仪测定血生化指标以及Elisa试剂盒测定骨钙素。结果振动刺激对失重性骨质疏松骨微结构的恢复及骨密度有明显的改善作用,变频振动对骨微结构的恢复及增加骨密度一定程度上要好于固定频率振动。结论对尾吊大鼠28天的不同频率振动刺激使松质骨骨矿物质以及空间上的结构进行了重构,导致尾吊大鼠骨质疏松模型中骨量的增加,骨密度的提高,对失重性骨质疏松有一定的防治作用,为临床上防治微重力环境下骨丢失提供了实验依据。

To study the therapeutic effect of frequency vibration on weightlessness- induced osteoporosis in the SD rats. Me thods Weightlessness- induced osteoporosis model was established using the mature method that was widely applied in the world. Forty 3-month-old SD male rats received 0. 5 g vibration at different frequencies（ 45 Hz,90 Hz,and 5- 90 Hz）. The rats were randomly divided into 5 groups,with 8 rats in each group. They were control group（ suspended only）,45 Hz group,90 Hz group,5- 90 Hz group,and suspended without vibration group. The vibration lasted for 10 min,once a day,5 days per week,and for 4 weeks.Rats in control group received no vibration but in the same noisy environment. Bone mass of the distal femur was measured using micro-CT. Blood biochemistry was measured with automatic analyzer. Osteocalcin was measured with ELISA method. Re sults Vibration stimulus is beneficial to the recovery of the microstructure of osteoporosis due to weightlessness and bone mineral density.The effect of variable frequency vibration is better than that of fixed frequency vibration. Conclusion Vibration reconstitutes cancellous bone mineral in this experiment,increases the bone mass and bone mineral density,prevents osteoporosis due to weightlessness,and provides the evidence for clinical prevention and treatment of weightlessness-induced bone loss.