miR-137靶向作用RUNX2调控MC3T3-E1细胞成骨分化

Targeting of RUNX2 by miR-137 and its regulation on MC3T3-E1 cells osteogenic differentiation

目的探讨miR-137对RUNX2的靶向作用及其对MC3T3-E1细胞成骨分化的影响。方法通过软件预测miR-137与成骨标志基因RUNX2的结合位点。检测MC3T3-E1细胞诱导成骨分化过程中miR-137的表达变化。转染miR-137 mimics或miR-137 inhibitor后,再诱导MC3T3-E1细胞成骨分化,检测成骨分化标志物(RUNX2、ALP、OPN、OCN及OSX)表达变化。结果经软件预测,miR-137与靶基因RUNX2有结合位点。MC3T3-E1细胞成骨分化过程中,miR-137表达下调。转染miR-137mimics的MC3T3-E1细胞成骨标志基因表达受到抑制,而转染miR-137 inhibitor组成骨标志基因呈不同程度表达上调。结论miR-137通过靶向作用RUNX2基因调控MC3T3-E1细胞成骨分化。

Objective To investigate the targeting of RUNX2 by miR-137 and its effect on MC3T3-E1 cell differentiation.Me thods The binding site of miR-137 and osteogenic marker gene RUNX2 was predicted by using an online prediction program.The expression level of miR-137 in MC3T3-E1 cells was detected during osteogenic differentiation. After transfection with miR-137 or miR-137 inhibitor, the MC3T3-E1 cells were induced to differentiate into osteoblasts, and the expression of osteogenic differentiation markers（ RUNX2,ALP,OPN,OCN and OSX） were detected. Re sults The RUNX2 gene as a potential target of miR-137 was identified using the miRNA target prediction program. miR-137 expression is down regulated during osteogenic differentiation of MC3T3-E1 cells. The expression level of osteogenic marker genes was inhibited in MC3T3-E1 cells after transfected with miR-137. However,the expression level of osteogenic marker genes was up-regulated after transfected with miR-137 inhibitor. Conclusion miR-137 regulates the osteogenic differentiation of MC3T3-E1 cells by targeting the RUNX2 gene.