三仁通便合剂的TLC及UPLC质量控制方法研究

Study on Quality Standard of Sanren Tongbian Mistura by TLC and UPLC

目的 建立三仁通便合剂质量控制方法。方法 利用薄层色谱法（TLC）及超高效液相色谱法（UPLC）对三仁通便合剂质量控制方法进行研究。结果 建立了方中大黄、当归两味饮片的TLC鉴别方法和苦杏仁苷的UPLC含量测定方法。苦杏仁苷在0.1104~1.1038mg范围内峰面积与进样量呈现良好的线性关系,r=0.9995,平均回收率为100.7%,RSD=1.75%。结论 本实验为三仁通便合剂的质量控制建立了稳定可行的方法。

OBJECTIVE To establish the quality standards of Sanren Tongbian mistura. METHODS Study on the quality standards of Sanren Tongbian mistura by TLC and UPLC. RESULTS Establish the standards of identifying Radix et Rhizoma and Radix Angelica Sinensis by TLC and determination of amygdalin by UPLC. The determination of amygdalin kept good linear relation in the content ranges of 0. 1104 - 1. 1038rag r = 0. 9995. The average recovery test of amygdalin was 100. 7%, RSD = 1.75%. CONCLUSION The established qualitative methods for Sanren Tongbian mistura were stable and feasible.