高效液相色谱法测定散结止痛颗粒中α-香附酮的含量测定

Determination of α-Cyperone in Sanjie Zhitong Granule by HPLC

目的 建立散结止痛颗粒中α-香附酮的含量测定方法。方法 高效液相色谱法（HPLC）测定α-香附酮的含量。采用Eclipse XDB-C18柱（250mm×4.6mm,5μm）色谱柱,以甲醇-水（80∶20）为流动相;流速为1.0m L·min-1,检测波长为254nm。结果 α-香附酮在2.01~40.2μg·m L-1线性关系良好,r=0.9994;该方法具有良好的精密度、稳定性和重复性,平均回收率98.84%,RSD为2.08%。结论 此方法准确可靠,操作简单,可用于测定散结止痛颗粒中α-香附酮的含量。

OBJECTIVE To establish a method for the determination of α-Cyperone in Sanjie Zhitong granules. METHODS The content of paeoniflorin was determined by HPLC. The Eclipse XDB-C18 （250mm × 4. 6mm, 5μm） was used, the mobile phase was Methanol-water（80：20）, the flow rate was 1.0mL . min-1, the detection wavelength was 254nm. RESULTS The linear range of α-Cyperone was within 2. 01 - 40. 21μg . mL-1 （ r = 0. 9994）. The average recovery was 98.84% （ RSD = 2.08% ）. CONCLUSION The method is accurate reliable and simple. It can be applied in the determination of α-Cyperone in Sanjie Zhitong granule.