蒙古族AD患者外周血IL-1和IL-18的检测及意义

The Test and Significance of IL-1,IL-18 in the Peripheral Blood of Mongolian Atopic Dermatitis Patients

目的研究IL-1、IL-18与蒙古族特应性皮炎(AD)患者发病的关系,探讨二者在蒙古族AD发病中的作用及意义。方法收集蒙古族AD患者100例(实验组)和正常对照者80例(对照组)血液标本,采用常规方法对外周血嗜酸性粒细(PBEC)行绝对计数,并应用酶联免疫吸附法(ElISA)测定研究对象IL-1(IL-1α和IL-1β)和IL-18的表达水平,对AD患儿进行搔抓、失眠、皮损面积、皮损严重度评分。采用统计学分析实验结果,得出变量间的相关性。结果 (1)实验组与对照组比较血清IL-1α值降低,IL-1β、IL-18与PBEC计数均升高(P<0.01)。(2)实验组血清IL-1β、IL-18与PBEC计数与病情严重程度均呈正相关(P<0.01)。(3)实验组血清IL-1α与PBEC计数与病情严重程度无显著相关性(P>0.05)。结论IL-1α、IL-1β、IL-18在蒙古族AD发病中起作用。

Objective To study the relationship between IL- 1 IL- 18 and Mongolian patients with atopic dermatitis （AD）, and to explore the role and significance of them about the pathogenesis of Mongolian atopic dermatitis. Methods Blood samples of 100 Mongolian AD patients（experimental group） and 80 normal cases （control group） were collected. The absolute counts of peripheral blood eosinophils （PBEC） were counted by routine method. The expression levels of IL- 1 （IL- 1α and IL- 1β） and IL- 18 were determined by en- zyme linked immunosorbent assay （EIlSA）. Evaluated the score of AD patients by scratching, insomnia, skin le- sion area and severity of lesion. Statistical analysis was used to analyze the correlation between variables. Results （1）Compared with the control group, the serum level of IL- 1α were decreased in the experimental group. The serum level of IL- 1β, IL- 18 and PBEC counts were increased（P 〈 0.01）. （2）The serum level of IL- 1β, IL- 18 and PBEC were positively correlated with the severity of the disease in the experimental group（P〈 0.01）. （3）The serum level of IL- la has no significance with the PBEC counts and severity of the disease（P 〉 0.05）. Conclusions IL - 1α, IL - 1β, IL - 18 play a role in the pathogenesis of Mongolian atopic dermatitis.