人肝细胞癌组织肿瘤相关巨噬细胞的浸润水平及其临床意义

Infiltration Level of Tumor-associated Macrophages in Human Hepatocellular Carcinoma and Its Clinical Significance

[目的]通过检测人肝细胞癌组织中肿瘤相关巨噬细胞的浸润水平和特点,探讨肿瘤相关巨噬细胞在肝癌发生、进展中的作用。[方法]以CD68分子为巨噬细胞标志,采用免疫组织化学法检测116例肝细胞癌肿瘤组织巨噬细胞的浸润水平和特点。[结果]肝细胞癌组织中CD68阳性表达率为94.8%(110/116),而癌旁组织为17.2%(20/116)。巨噬细胞的浸润水平在不同TNM分期、是否存在血管侵袭、淋巴结转移及微卫星病灶分组间均有统计学差异(P<0.05),与患者预后呈负相关(P=0.001)。[结论]人肝细胞癌组织肿瘤相关巨噬细胞呈高浸润状态,且浸润水平与多项临床病理特征和预后密切相关,提示其可能参与了肝癌的发生、进展。

[Objective] To examine the level and characteristics of tumor-associated macrophage in human hepatocellular carcinoma（HCC） and to evaluate its role in the progression of HCC.[Methods] Macrophages marked by CD68 molecule in liver tissues from 116 patients with HCC were examined by immunochemistry staining. [Results] About 94.8%（110/116） samples were positive for CD68 in these tumor specimens,while 17.2%（20/116） in adjacent tissues. Significant differences of macrophages＇ infiltration were found among early and late TNM stages and exists or not of vascular invasion,lymph metastasis and microsatellite lesions（P0.05）. Meanwhile,the levels of macrophages in tumor tissues were negatively correlated with postoperative prognosis（P=0.001）.[Conclusions] Macrophages are shown high infiltration levels in HCC tissues and closely relate to several clinical parameters,tumor staging and survival of HCC. Therefore,TAMs might involve in the mechanisms of HCC.