钴60辐照制备贾第鞭毛虫孢囊标准体及其保存条件

Preliminary study on preservation and preparation of Giardia standard body by ^(60)Co irradiation

目的研究钴60灭活制备贾第鞭毛虫孢囊标准体的方法,并对孢囊保存条件进行初探。方法选取不同剂量(0~1200 Gy)钴60-γ射线对贾第鞭毛虫活体孢囊进行辐照,采用荧光染色方法判定辐照灭活效果,同时将灭活后的贾第鞭毛虫置于2.5%重铬酸钾中4℃保存,观察其在灭活后5个月内的变化。结果当钴60-γ射线辐照剂量为900 Gy时灭活率达到91.44%±1.64%,此时贾第鞭毛虫卵囊完整,抗原性保持完好,符合《生活饮用水卫生标准》要求。保存期为4个月时,细胞总数量、灭活率、细胞形态等均符合实验需求。结论 900 Gy钴60-γ射线辐照可作为贾第鞭毛虫孢囊灭活的有效方法,贾第鞭毛虫在2.5%重铬酸钾中4℃低温保存期限为4个月。

Objective Preliminary study on preparation methods of Giardia standard body by60Co devitalized and the preservation conditions.Methods Giardia irradiated by different doses of60Co 0-1200 Gy and assessed by immunofluorescence assay.In the same time,the inactivated Giardia were preserved in 2.5% K2Cr2O7 at 4℃.Then the Giardia by immunofluorescence assay was observed over the next 5 months.Results After treatment with 900 Gy of60Co radiation,91.44% ± 1.64% of the cysts were inactivated.Moreover,indicators of cysts meet the requirements.After stored at 4 ℃ for 4 months,the number of total cysts,inactivation ratio and the morphology of cysts were meet the requirement.Conclusion It is an effective method to inactivate Giardia cysts with 900 Gy irradiation of60Co.And Giardia cysts can be preserved in 2.5% K2Cr2O7 at 4 ℃ for 4months.