固定化酶催化椰子油水解工艺研究

Hydrolysis of coconut oil catalyzed by immobilized lipase

利用水浴振荡辅助固定化酶进行椰子油水解工艺研究。比较3种固定化酶水解效率并筛选。以反应体系的酸值为响应值,以温度、p H、加酶量、水油质量比为影响因素,采用单因素实验结合Box-Behnken实验,建立反应体系酸值的二次多项式回归方程,通过响应面分析椰子油的最佳固定化酶水解条件,并在优化条件下研究酶的回收及操作稳定性。结果表明,温度80℃、p H 5.5、Lipozyme 435加酶量6.51%和水油质量比1.08∶1为固定化酶催化椰子油水解的最佳工艺条件,该条件下反应20 h,椰子油的水解率为94.80%,重复使用10次后,酶活仍能保留86%,稳定性较好。

In water bath oscillator,the hydrolysis of coconut oil catalyzed by immobilized lipase was stud- ied. The hydrolysis effects of three kinds of immobilized lipases were compared and the best immobilized lipase was screened. With acid value of reaction system as response value, and temperature, pH, dosage of enzyme and mass ratio of water to oil as factors, quadratic polynomial regression equation of acid value was established by single factor experiment and Box -Behnken experiment, and the hydrolysis conditions of coconut oil were optimized by response surface methodology. Also the recycling and operation stability of the lipase were studied under the optimal conditions. The results showed that the optimal hydrolysis condi- tions of coconut oil were obtained as follows： temperature 80~C, pH 5.5, dosage of Lipozyme 435 6.51% and mass ratio of water to oil 1.08： 1. Under these conditions,the hydrolysis rate was 94. 80% after reaction for 20 h and the enzyme activity retained 86% after ten times recycling,showing a good stability.