二穗短柄草愈伤组织的诱导及农杆菌介导的遗传转化技术在本科实验教学中的应用

The Application of Callus Induction and Agrobacterium-Mediated Transformation Technology of Brachypodium distachyon in the Undergraduate Experimental Course

植物胚性愈伤组织诱导及再分化技术对植物培育及良种品质的选育有重要作用,但在目前高校本科实验教学中缺少相关内容。作者选用二穗短柄草这一单子叶模式植物作为实验材料,将二穗短柄草胚发育观察、愈伤组织诱导与再分化及农杆菌介导的遗传转化融合在一个实验中,并且对该实验做了细致的实践,设计了一整套适合本科实验教学的方案。该实验直观、系统地展示了愈伤组织诱导和农杆菌介导的遗传转化技术,不仅实验结果生动形象,而且能拓宽学生的实验技能和知识领域。该教学实验设计还为愈伤组织诱导及农杆菌介导的遗传转化技术在本科实验课程中的推广提供了有益建议。

The technology of embryogenic callus induction and regeneration plays an important role in plant breeding and selection of improved variety. However, undergraduate experimental courses lack the relevant content. In this project, we choose model monocot plant Brachypodium distachyon as experimental material, and integrated phenotype observation ofBrachypodium distachyon, callus induction and redifferentiation, and Agrobac- terium-mediated transformation into one project. We did practice in detail, and then designed a series of suitable experimental procedures for undergraduate courses. This experiment included technologies of callus induction and genetic transformation, and was designed to ensure attending students to get positive results, improving their experi- mental skills, and broaden their knowledge. We furthermore provided valuable suggestions for the popularization of this project in undergraduate experimental course.