新疆加工番茄马铃薯Y病毒株系鉴定

Differentiation and identification of Potato virus Y strains infecting processing tomato cultivars in Xinjiang,China

马铃薯Y病毒(Potato virus Y,PVY)是世界范围内对茄科属植物造成影响的重要病毒,为了解PVY对新疆加工番茄的危害,本文采集205份加工番茄样品进行血清学检测,结果 PVY^O、PVY^(O\N\C)、PVY^N检出率分别为20%、18.05%和0%,且PVY不同株系总检出率达24.9%。根据血清学检测结果,在苋色藜(Chenopodium amaranticolor)上进行单斑分离获得2个PVY分离物S1-10、S2-12,其基因组序列分析结果显示,S1-10分离物是PVY^O与PVY^N的重组体,属于PVY^(N∶O)株系,S2-12属于PVY^O株系。本研究表明新疆加工番茄上的PVY主要为PVY^(N∶O)和PVYO株系。

Potato virus Y （PVY） causes severe yield loss as well as quality degradation globally by infecting plants of the Solanaceae family. In this study, 205 processing tomato samples from Xinjiang province were col- lected, followed by serological analysis. Result showd that 24.9% of the samples were infected by PVY, among which 20% and 18.05% were found to contain PVYO and PVYO／N／c strains, respectively, whereas PVYN was not detected in all the tomato samples. Two PVY isolates （S1-10 and S2-12） were obtained from the infected tomato plants and further biologically differentiated through single local lesion transfer on the Chenopodium amarantico- lor leaves. Sequence analysis indicated that SI-10 was a recombinant between PVYO and PVYN and designated as PVYNN：O. S2-12 shared high sequence similarity with the swain of PVYO. These results demonstrated that PVYNN：O and PVYO were the dominant strains infecting processing tomato cultivars in Xinjiang.