5种木兰科植物的DNA-C值及倍性分析

Analysis of DNA C-value and Ploidy of Five Species in Magnoliaceae

以紫玉兰为内标或外标,利用流式细胞术测定了杂交马褂木(Liriodendron chinense×tulipifera)、深山含笑(Michelia maudiae)、长蕊含笑(Michelia longistamina)、广玉兰(Magnolia grandiflora)和二乔玉兰(Magnolia soulangeana)5种木兰科植物的DNA-C值及倍性。结果表明,两种含笑属植物及杂交马褂木均为二倍体,广玉兰为六倍体,二乔玉兰为四倍体,5种植物的C值各不相同,最大的是广玉兰,最小的是杂交马褂木,它们的C值大小属于小到中等水平。

In this study,the C-value and ploidy of five species in Magnoliaceae was estimated by flow cytometry using Magnolia liliiflora as the reference. The results showed that the two species of Michelia are diploid,Magnolia grandiflora is hexaploid,and M. soulangeana is tetraploid. The C-Values of five species are different from each other. The C-value of M. grandiflora is the largest among the five species investigated,while the C-value of Liriodendron chinense × tulipifera is the smallest. The sizes of C-values of the five species are small to medium level.