低温胁迫下外源脱落酸对高山离子芥抗氧化酶和渗透调节物质的影响

Exogenous ABA Affected on Antioxidant Enzymes and Osmotic Adjustment in Choraspora bungeana Under Low Temperature Stress

以高山离子芥(Chorispora bungeana)试管苗为实验材料,研究了低温冷害(4℃)、低温冻害(-4℃)和临界温度(0℃)胁迫下外源ABA对抗氧化酶及渗透调节物质的影响。结果表明在4和-4℃胁迫下阴性对照组中SOD和CAT活性高于空白对照组;POD活性在4℃胁迫下高于空白对照组;APX活性在4和-4℃胁迫的晚期升高。与此同时,阴性对照组中膜的相对电导率增加、脯氨酸、可溶性糖含量降低。0℃胁迫下阴性对照组中POD活性降低;APX活性呈波动式变化;脯氨酸和可溶性糖含量分别在72和48 h时高于空白对照组;而可溶性蛋白含量低于空白对照组。添加外源ABA处理后4、-4和0℃胁迫下POD、SOD、CAT和APX活性较阴性对照组均得到不同程度增加;ABA的处理显著降低了细胞膜的相对电导率,提高脯氨酸,可溶性糖和可溶性蛋白的含量。

Using plantlets of Choraspora bungeana as material,researched the effect of exogenous ABA on antioxidant enzyme and osmotic regulation substances under chilling（ 4℃）,freezing（- 4℃） and critical temperature（ 0℃） were studied. The results showed the activities of SOD and CAT in negative control group were higher than blank control group under 4℃ and- 4℃. In negative control group the POD activity was higher than blank control group under 4℃. The APX activity increased at the late period of 4℃ and- 4℃treatment in negative control group. Meanwhile the relative conductivity increased but proline and soluble sugar decreased in negative control group. At 0℃,in negative control group the POD activity decreased and the APX activity changed with fluctuation. The content of proline and soluble sugar was higher than blank control group at 72 h and 48 h respectively and soluble protein was lower than blank control group under 0℃. Exogenous ABA treatment,increased the antioxidant enzymes activities with different extent under low temperature stress. For the osmotic regulation substances,exogenous ABA treatment decreased the relative conductivity and increased the content of proline,soluble sugar and soluble protein.