普通小麦TaNAC1基因的表达及在拟南芥中的遗传转化

Expression of Ta NAC1 Gene in Common Wheat and Genetic Transformation in Arabidopsis

NAC类转录因子是植物特有的转录因子家族,在调节植物生长发育及逆境胁迫应答反应中起着重要作用。本文从普通小麦幼叶中获得了一个编码NAC结构域的转录因子基因,命名为Ta NAC1;氨基酸序列分析表明,Ta NAC1具有典型的NAC类转录因子所具有的五个亚结构域,隶属于NAC类转录因子的ATAF亚类;亚细胞定位实验表明,Ta NAC1蛋白在细胞核内表达;转录水平上,Ta NAC1基因的表达受到PEG、ABA、低温及高盐等非生物胁迫条件的诱导;将Ta NAC1转化拟南芥后,与野生型比较发现,Ta NAC1基因的过量表达会使转基因植株出现叶片发育畸形且生长缓慢,植株矮化及茎部融合等表型,表明Ta NAC1基因可能在参与小麦叶片及茎的发育中起着重要的调控作用。

NAC-type transcription factor is one of the plant-specific gene families,which plays key roles in regulating the development and response to environmental stress in plants. A NAC-type transcription factor,designated Ta NAC1,was cloned from leaves in wheat. The encoding sequence of Ta NAC1 contained five typical NAC conserved domains and belonged to ATAF sub-type NAC family. The Ta NAC1 protein was located in the nucleus by transient expression in onion epidermis cell. The transcriptional expression was induced by environmental stresses such as ABA,PEG,low temperature and high salt. Phenotypic analysis in the transgenic Arabidopsis showed that over-expression of Ta NAC1 caused abnormal leaves,dwarfism and fused stem in transgenic Arabidopsis. Ta NAC1 maybe play important roles in regulation during the development of leaves and stems in wheat.