蛇床子催眠活性组分对小鼠镇静催眠、学习记忆及宿醉反应的影响

Effects of Hypnotic Active Constituent of Cnidium monnieri on Sedation and Hypnosis,Learning and Memory,Hangover of Mice

目的:研究蛇床子催眠活性组分(CHC)对小鼠的镇静催眠、学习记忆及宿醉反应的影响。方法:取小鼠60只,随机分为空白对照组(2%聚山梨酯80溶液)、地西泮组(6 mg/kg)和CHC高、中、低剂量组(520、260、130 mg/kg),每组12只,每天ig相应药物2次。观察给药1 d后各组小鼠的入睡潜伏期和睡眠持续时间、睡眠后自主活动次数和站立次数、避暗实验的避暗潜伏期和错误次数;给药16 d后各组小鼠进行定位航行实验和空间探索实验。结果:与空白对照组比较,地西泮组和CHC各剂量组小鼠睡眠持续时间均延长、睡前和醒后站立次数均减少;地西泮组小鼠睡前错误次数增加,醒后活动次数减少,逃避潜伏期延长,目标时间缩短,目标路程占比减少;CHC高剂量组小鼠睡前避暗潜伏期延长,以上差异均有统计学意义(P<0.05或P<0.01)。与地西泮组比较,CHC中、高剂量组小鼠睡眠持续时间均延长,醒后活动次数均增加;CHC低剂量组小鼠睡前活动次数减少;CHC各剂量组小鼠睡前错误次数均减少,以上差异均有统计学意义(P<0.05或P<0.01)。与睡前比较,空白对照组小鼠醒后避暗潜伏期延长,CHC各剂量组小鼠醒后活动次数均增加、醒后站立次数均减少、醒后避暗潜伏期延长(除中剂量组外),以上差异均有统计学意义(P<0.05)。其余指标差异均无统计学意义(P>0.05)。结论:CHC对小鼠有镇静催眠作用,但对其学习记忆几乎无影响,也没有宿醉反应。

OBJECTIVE：To study the effects of hypnotic active constituent of Cnidium monnieri（CHC）on sedation and hypnosis,learning and memory,hangover of mice.METHODS：60 mice were randomly divided into blank control group（2% Tween80 solution）,diazepam group（6 mg/kg）and CHC high-dose,medium-dose and low-dose groups（520,260,130 mg/kg）,with12 mice in each group.They were given relevant medicine intragastrically twice a day.The sleep latency and sleep duration,the times of autonomic activities and standing times after sleep,escape incubation period and the number of errors in step through test were observed in each group 1 d after medication.The place navigation test and spatial probe test were conducted 16 day after medication.RESULTS：Compared with blank control group,sleep duration of mice was prolonged in diazepam group and CHC groups while standing times before and after sleep were decreased;in diazepam group,the number of errors was increased before sleep,while activity times was decreased after sleep;escape incubation period was prolonged,while target time was shortened;the ratio of target distance was decreased;escape incubation period of mice was prolonged in CHC high-dose group,with statistical significance（P〈0.05 or P〈0.01）.Compared with diazepam group,sleep duration of mice was prolonged in CHC medium-dose and high-dose group,and activity times after sleep were increased;activity times before sleep were decreased in CHC low-dose group;the number of errors before sleep were decreased in CHC groups,with statistical significance（P〈0.05 or P〈0.01）.Compared with before sleep,escape incubation period of blank control group was prolonged;activity times after sleep of CHC groups were increased while standing times after sleep were decreased;escape incubation period after sleep was prolonged（except for medium-dose group）,with statistical significance（P〈0.05 or P〈0.01）.There was no statistical significance in other indexes（P〉0.05）.CONCLUSIONS：CHC shows sedation and hypnotic effect in mice,but has no effect on learning and memory and doesn’t induce hangover response.