封丘产金银花不同提取物的体外抗氧化、抗凝血及α-葡萄糖苷酶抑制活性研究

Study on in vitro Antioxidant,Anticoagulant and α-glucosidase Inhibitory Activities of Different Extracts of Lonicerae japonicae from Fengqiu

目的:考察封丘产金银花不同提取物的体外抗氧化、抗凝血及α-葡萄糖苷酶抑制活性。方法:以30%乙醇提取金银花得总浸膏,用水分散后依次用乙酸乙酯、正丁醇萃取得相应部位萃取物。采用1,1-二苯基-2-苦肼基(DPPH)自由基、2,2′-联氮基-双-(3-乙基苯并噻唑啉-6-磺酸)二氨盐(ABTS)自由基清除试验和Fe^(3+)还原/抗氧化能力试验考察金银花不同提取物的体外抗氧化活性大小[分别以半数清除浓度(IC_(50))和Trolox当量反映];并考察4种不同提取物的体外凝血和抑制α-葡萄糖苷酶的活性强弱。结果:4种提取物中,乙酸乙酯部位抗氧化能力最强,ABTS、DPPH自由基的IC50分别为9.22、25.23μg/ml,Trolox当量为(2 480.80±5.51)μmol/g;水提物部位促凝血活性最强、正丁醇部位抗凝血活性最强,血浆复钙时间分别为(210.28±2.19)、(149.20±1.25)s;总浸膏、乙酸乙酯部位均对α-葡萄糖苷酶活性有较强的抑制作用,复筛IC_(50)分别为378.61、451.45μg/ml。结论:封丘产金银花有较好的体外抗氧化、抗凝血及α-葡萄糖苷酶抑制活性,可进一步开发。

OBJECTIVE：To investigate in vitro antioxidant,anticoagulant and α-glucosidase inhibitory activities of different extracts of Lonicerae japonicae from Fengqiu.METHODS：The total extract was obtained from L.japonicae by extraction with 30%ethanol,and dispersed in water,from which the extracts of corresponding parts were obtained after extraction successively with trichloromethane and ethyl acetate.The in vitro antioxidant activity of different extracts from L.japonicae [reflected by IC50,Trolox equivalent value] was investigated by 1,1-diphenyl-2-picrylhydrazyl（DPPH）radical,2,2′-azinobis-（3-ethylbenzthiazoline-6-sulphonate）（ABTS）radical scavenging test and Fe3＋reduction and antioxidant capacity test.The α-glucosidase inhibitory activity and coagulant activity of 4 extracts were also studied in vitro.RESULTS：The ethyl acetate extract of L.japonicae had the highest antioxidant activity among 4 extracts,IC50 of ABTS and DPPH were 9.22,25.23 μg/ml and Trolox equivalent value was（2 480.80 ±5.51）μmol/g.Procoagulant activity of water extract and anticoagulation activity of n-butyl alcohol extract were the highest;the plasma recalcification time were（210.28±2.19）s and（149.20±1.25）s.Both total extract and ethyl acetate part inhibited the activity of α-glucosidase,and IC50 of re-screening were 378.61,451.45 μg/ml.CONCLUSIONS：L.japonicae from Fengqiu have good antioxidant,anticoagulant and α-glucosidase inhibitory activities in vitro,and could be further developed and studied.