壮药痔疮熏洗液的止血、镇痛、抗炎和抗菌作用研究

Study on the Hemostasis,Analgesic,Anti-inflammatory and Anti-bacterial Effects of Zhuang Medicine Hemorrhoids Fumigant

目的:考察壮药痔疮熏洗液的止血、镇痛、抗炎和抗菌作用。方法:将KM小鼠(或SD大鼠)按体质量随机分为模型对照组、复方荆芥熏洗剂组[阳性药物,0.56 g(生药)/kg]和痔疮熏洗液低、中、高剂量组[2.40、4.80、9.60 g(生药)/kg],每组10只,外用给药。分别通过测定小鼠断尾出血时间、0.6%冰醋酸致小鼠扭体次数、二甲苯致小鼠耳肿胀度和角叉菜胶致大鼠足跖肿胀度考察其止血、镇痛和抗炎作用。以肉汤二倍稀释法测定其对绿脓假单胞菌(PA)、表皮葡萄球菌(SE)、大肠埃希菌(EC)、白色念珠菌(MA)、金黄色葡萄球菌(SA)、乙型副伤寒沙门菌(SPB)、枯草芽孢杆菌(BS)、肺炎克雷伯菌(KP)的最低抑菌浓度(MIC)及最低杀菌浓度(MBC)。结果:与模型对照组比较,各给药组小鼠的断尾出血时间缩短、扭体次数减少、耳肿胀度降低,各给药组大鼠足跖肿胀度降低,差异均有统计学意义(P<0.05或P<0.01);痔疮熏洗液对PA、SE、EC、MA、SA、SPB、BS、KP的MIC分别为0.045、0.091、0.091、0.181、0.091、0.091、0.091、0.181 g/ml,MBC分别为0.091、0.181、0.181、0.363、0.091、0.181、0.181、0.181 g/ml。结论:痔疮熏洗液具有明显的止血、镇痛、抗炎和抗菌作用。

OBJECTIVE：To investigate the hemostasis,analgesic,anti-inflammatory and anti-bacterial effect of Zhuang medicine Hemorrhoids fumigant.METHODS：KM mice（or SD rats） were randomly divided into model control group,Compound schizonepeta lotion group [positive drug,0.56 g（crude drug）/kg] and Hemorrhoids fumigant low-dose,medium-dose and highdose groups [2.40,4.80,9.60 g（crude drug）/kg] according to body mass,with 10 rats or mice in each group,for external administration.The hemostasis,analgesic and anti-inflammation effects of Hemorrhoids fumigant were investigated through determining tail bleeding time,0.6% acetic acid-writhing times and xylene-induced ear swelling of mice and carrageenin-induced paw swelling of rats.The broth two times dilution method was used to determine minimal inhibitory concentration（MIC）and minimum bactericidal concentration（MBC）of Hemorrhoids fumigant to Pseudomonas aeruginosa（PA）,Staphylococcus epidermidis（SE）,Escherichia coli（EC）,Monilia albicans（MA）,Staphylococcus aureus（SA）,Salmonella paratyphi B（SPB）,Bacillus subtilis（BS）and Klebsiella pneumoniae（KP）.RESULTS：Compared with model control group,the tail bleeding time,writhing times and the degree of ear swelling in mice were decreased in treatment groups,and the degree of paw swelling in rats was decreased in treatment groups,with statistical significance（P〈0.05 or P〈0.01）.MIC of Hemorrhoids fumigant to PA,SE,EC,MA,SA,SPB,BS,KP were 0.045,0.091,0.091,0.181,0.091,0.091,0.091,0.181 g/ml,respectively;corresponding MBC were 0.091,0.181,0.181,0.363,0.091,0.181,0.181,0.181 g/ml,respectively.CONCLUSIONS：Hemorrhoids fumigant shows obvious hemostasis,analgesic,anti-inflammatory and anti-bacterial effects.