常压室温等离子体快速诱变筛选高产纤维素酶生产菌株

Rapid Breeding and Screening of A High Cellulase-producing Mutant by Atmospheric Room Temperature Plasma

以解淀粉芽孢杆菌(Bacillus amyloliquefaciens strain)JC-1为出发菌株,采用常压室温等离子体诱变系统(Atmospheric and room temperature plasma,ARTP)进行诱变,根据刚果红透明圈大小以及96孔板高通量筛选法筛选出产纤维素酶活力高的突变菌株。选育出一株遗传稳定性良好的突变菌株,命名为T-16菌株。结果表明:该菌株产纤维素酶活力达到1.759 U/m L,比出发菌株提高了41.8%。对突变菌株T-16进行发酵特性研究,发现突变菌株T-16比出发菌株产酶时间提前了8 h。

A novel mutation approach, the atmospheric room temperature plasma （ARTP）, was used to treat Bacillus amyloliquefaciens strain JC-1 for the breeding of high cellulose productivity. The mutant with high cellulose productivity was quickly screened based on the ratio of transparent circle on Congo red screening plate and cellulose productivity on 96-well plate. The obtained mutant T-16 exhibited good genetic stability and cellulose productivity reached 1. 759 U/mL, which was 41.8% higher than that of the original strain. The fermentation characteristics of mutant strain T-16 were studied and the results showed that cellulose production time advanced 8 hours compared with the original strain.