环介导等温扩增法在常见性病病原体检测中的应用效果分析

Effect analysis of loop-mediated isothermal amplification for common STD pathogen detection

目的:分析环介导等温扩增法在常见性病病原体检测中的应用效果。方法:选取我院2013年11月至2015年2月收治的性病患者126例为研究对象,对生殖道分泌物进行检测,同时采用LAMP法、涂片检测和细菌培养方法,分析阳性检测结果和敏感性、特异性。结果:取扩增产物进行酶切片段检测,酶切片段与理论预计相符合,Alu I酶切片段包括139bp、70bp,Tai I主要片段包括196bp、160bp、144bp,环介导等温扩增法和PCR检测阳性率无明显差异(P>0.05),环介导等温扩增法、PCR检测HPV敏感性无统计学差异(P>0.05),环介导等温扩增法检测CT、淋球菌和TP敏感性均高于PCR检测。结论:常见性病病原体采用环介导等温扩增法检测快速、置管、便捷,敏感性和特异性都较高,无需特殊仪器,具有使用价值。

Objectives： To analyze the application effect of loop-mediated isothermal amplification for common STD pathogen detection and provide reference for clinical test. Methods： 146 patients with venereal disease in hospital from November 2013 to February 2015 were selected. Patients＇ genital secretions were detected according to loop-mediated isothermal amplification method and PCR. The positive test results,sensitivity and specificity were compared. Results： Amplification product fragment was detected. Fragment and the theoretical expectation were consistent. Alu I fragment comprises were 139 bp and 70 bp. Tai I primary segment comprises were 196 bp,160bp and 144 bp. Positive rate of loop-mediated isothermal amplification method and the PCR test had no significant difference（ P〉0. 05）. Sensitivity of sexually transmitted pathogens of loop-mediated isothermal amplification method and PCR had no significant difference（ P〉0. 05）. The sensitivity of Ct,Neisseria gonorrhoeae and TP by loop-mediated isothermal amplification was higher than that of PCR detection. Conclusion： Loop-mediated isothermal amplification in the detection of common sexually transmitted pathogens is fast,cathetering and convenience with high sensitivity and specificity,which doesn＇t need special equipment and is worth of promotion.